Expression and localization of a novel phosducin-like protein from amphioxus Branchiostoma belcheri
文献类型：CNKI期刊论文
| 作者 | 萨仁高娃; Zhao YG(赵永刚) |
| 发表日期 | 2009-05-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | amphioxus B. belcheri Amphi-PhLP northern blotting in situ hybridization cellular localization |
| 英文摘要 | A full length amphioxus cDNA, encoding a novel phosducin-like protein (Amphi-PhLP), was identified for the first time from the gut cDNA library of Branchiostoma belcheri. It is comprised of 1 550 bp and an open reading frame (ORF) of 241 amino acids, with a predicted molecular mass of approximately 28 kDa. In situ hybridization histochemistry revealed a tissue-specific expression pattern of Amphi-PhLP with the high levels in the ovary, and at a lower level in the hind gut and testis, hepatic caecum, gill, endostyle, and epipharyngeal groove, while it was absent in the muscle, neural tube and notochord. In the Chinese Hamster Ovary (CHO) cells transfected with the expression plasmid pEGFP-N1/Amphi-PhLP, the fusion protein was targeted in the cytoplasm of CHO cells, suggesting that Amphi-PhLP is a cytosolic protein. This work may provide a framework for further understanding of the physiological function of Amphi-PhLP in B. belcheri. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by the Pilot Projects of Knowledge Innovation Project of Chinese Academy of Sciences (No. KZCX2-YW-211-03 and MGE2008KG06) |
| 卷 | v.27期:02页:142-148 |
| 语种 | 英文; |
| 分类号 | Q78 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/192583]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.DepartmentofMarineBiology,OceanUniversityofChina 2.KeyLaboratoryofMarineGeologyandEnvironment,InstituteofOceanology,ChineseAcademyofSciences 3.ChinaAnimalHealthandEpidemiologyCenter |
| 推荐引用方式 GB/T 7714 | 萨仁高娃,Zhao YG. Expression and localization of a novel phosducin-like protein from amphioxus Branchiostoma belcheri. 2009. |
入库方式： OAI收割
来源：海洋研究所
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