Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype,allozyme and RAPD analyses
文献类型：CNKI期刊论文
| 作者 | You F(尤锋); Wang W(王伟); Xu DD(徐冬冬); Zhu XP(朱香萍); Ni J(倪静); Wu ZH(吴志昊); Xu YL(徐永立); Wang XC(王新成); Zhang PJ(张培军) |
| 发表日期 | 2009-05-15 |
| 出处 | Chinese Journal of Oceanology and Limnology
 |
| 关键词 | artificial hybridization Paralichthys olivaceus (Temminck et Schlegel) Kareius bicoloratus (Basilewsky) karyotype allozyme RAPD |
| 英文摘要 | The hybrid between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus was produced by artificial insemination of olive flounder eggs with stone flounder sperm. Sinistral and dextral are two types of hybrid progeny after metamorphosis. Karyotypes of both hybrid flounders are the same as those of the two parental species. Of the 22 loci examined from 12 allozymes, 12 confirmed hybridization of the paternal and maternal loci in hybrids and no difference was found in allozyme patterns of sinistral and dextral hybrid fishes. RAPD patterns of these specimens were also studied with 38 primers selected from 104 tested. Among them, the PCR products of 30 primers showed hybridization of the paternal and maternal bands. Genetic variation between hybrids and their parental stocks was analyzed by RAPD using 10 of the above 38 primers. The average heterozygosity and genetic distance were calculated. The results suggested that the filial generation could inherit a little more genetic materials from paternal fish than that from maternal fish. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | Supported by National Natural Science Foundation of China (No. 30571445) ; National High-Technology Research and Development Program (863 Program, No. 2006AA10A404) ; Project from the Ministry of Science and Technology of China (No. 2006DKA30470-017) |
| 卷 | v.27期:02页:135-141 |
| 语种 | 英文; |
| 分类号 | S917.4 |
| ISSN号 | 0254-4059 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/192596]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.InstituteofOceanology,ChineseAcademyofSciences 2.QingdaoAgriculturalUniversity |
| 推荐引用方式 GB/T 7714 | You F,Wang W,Xu DD,et al. Hybrids between olive flounder Paralichthys olivaceus and stone flounder Kareius bicoloratus: karyotype,allozyme and RAPD analyses. 2009. |
入库方式： OAI收割
来源：海洋研究所
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