Inheritance of 15 microsatellites in the Pacific oyster Crassostrea gigas:segregation and null allele identification for linkage analysis
文献类型:CNKI期刊论文
作者 | Li L(李莉); Guo XM(郭希明); Zhang GF(张国范) |
发表日期 | 2009-02-15 |
出处 | Chinese Journal of Oceanology and Limnology
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关键词 | Crassostrea gigas microsatellite null allele genetic mapping |
英文摘要 | Microsatellites were screened in a backcross family of the Pacific oyster, Crassostrea gigas. Fifteen microsatellite loci were distinguishable and polymorphic with 6 types of allele-combinations. Null alleles were detected in 46.7% of loci, accounting for 11.7% of the total alleles. Four loci did not segregate in Mendelian Ratios. Three linkage groups were identified among 7 of the 15 segregating loci. Fluorescence-based automated capillary electrophoresis (ABI 310 Genetic Analyzer) that used to detect the microsatellite loci, has been proved a fast, precise, and reliable method in microsatellite genotyping. |
文献子类 | CNKI期刊论文 |
资助机构 | Supported by the National Natural Science Foundation of China (NO.40730845, 39825121) |
卷 | v.27期:01页:76-81 |
语种 | 英文; |
分类号 | S917.4 |
ISSN号 | 0254-4059 |
源URL | [http://ir.qdio.ac.cn/handle/337002/192692] ![]() |
专题 | 中国科学院海洋研究所 |
作者单位 | 1.HaskinShellfishResearchLaboratory,InstituteofCoastalandMarineSciences,RutgersUniversity 2.KeyLaboratoryofExperimentalMarineBiology,InstituteofOceanology,ChineseAcademyofSciences |
推荐引用方式 GB/T 7714 | Li L,Guo XM,Zhang GF. Inheritance of 15 microsatellites in the Pacific oyster Crassostrea gigas:segregation and null allele identification for linkage analysis. 2009. |
入库方式: OAI收割
来源:海洋研究所
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