中国对虾非包涵体型杆状病毒核衣壳的分离纯化
文献类型：CNKI期刊论文
| 作者 | 徐洪涛,朴春爱,王雷,杨朵,屈建国,张宝云,候云德 |
| 发表日期 | 2000-06-30 |
| 出处 | 病毒学报
 |
| 关键词 | 中国对虾 杆状病毒 氯化铯密度梯度 分离纯化 电子显微镜 |
| 英文摘要 | An effective procedure for isolation and purification of nucleocapsids of Penaeus chinensis non-occluded baculovirus (PcNOBV) which has destroyed the Chinese shrimp industry since 1993 was described. Gill, stomach, gut and cuticle epidermis under exoskeleton were excised from cultured P. chinensis diseased with typical white spot syndrome and homogenized in liquid nitrogen with TNE buffer containing PMSF and β-ME. The homogenized mixture was filtered through a 0.45μm millipore filter membrane to remove cell debris and ultracentrifuged to pellet the remaining material.The pellet was suspended in PMTNE buffer and laid onto a handmade CsCl gradient. An obvious viral band was observed in the middle of the gradient. Large amounts of virus nucleocapsids were visualized under electron microscope consistently corresponding to the milk-colored viral band. The viral envelope was all lost after purification. The nucleocapsid was bacilliform averaging 80±13nm×380±24nm in size. The negatively stained PcNOBV nucleocapsids revealed 13-16 conspicuous stripes located periodically perpendicular to the longitudinal axis of the nucleocapsids. Six to seven capsomers of 9 nm in diameter were visualized on each side of the stripe. |
| DOI标识 | 10.13242/j.cnki.bingduxuebao.001235 |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | 中国科学院海洋研究所EMBL开放课题 ; 海洋863!( 819-0 2 -0 5 ) ; 病毒基因工程国家重点实验室客座课题 |
| 期 | 02页:91-93 |
| 语种 | 中文; |
| 分类号 | Q936 |
| ISSN号 | 11-3424/R |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/197515]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.中国科学院海洋研究所!山东青岛266071 2.中国预防医学科学院病毒学研究所!北京100052 3.西安医科大学!陕西西安710061 4.中国预防医学科学院病毒学研究? |
| 推荐引用方式 GB/T 7714 | 徐洪涛,朴春爱,王雷,杨朵,屈建国,张宝云,候云德. 中国对虾非包涵体型杆状病毒核衣壳的分离纯化. 2000. |
入库方式： OAI收割
来源：海洋研究所
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