海湾扇贝个体间单向人工授精的分子生物学验证
文献类型：CNKI期刊论文
| 作者 | 孙博; 刘晓; 张国范; 郑怀平; 郭希明 |
| 发表日期 | 2006-10-30 |
| 出处 | 水产学报
 |
| 关键词 | 海湾扇贝 人工授精 杂交 家系 RAPD 分子标记 |
| 英文摘要 | Hybrid family of Argopecten irradians irradians was created by fertilization between two northern bay scallop individuals.Two families were analyzed in this study.The first family,P_a-P_b,is a pair mating between two scallops named P_a and P_b,while the second one crossed by individuals of Y_1 and P_0.Marker inheritance and segregation were studied in the 10 progenies of each family by randomly amplified polymorphic DNA(RAPD) analysis.102 RAPD primers were first screened by parental animals of both families.Only the primers with polymorphisms between the two parental animals in each family were selected for further analysis.In both families,parents and 10 progeny were analyzed with selected primers.In family P_a-P_b,a total of 122 bands generated from 12 selected primers.37 of them were polymorphic between two parents.The maternal P_a of this family had 17 molecular markers while paternal P_b had 20 markers.In Y_1-P_0,95 bands were produced by 10 selected primers.32 bands were polymorphic between maternal Y_1 and paternal P_0,who had 17 and 15 molecular markers respectively.In both families,each progeny analyzed in this study had at least 8 maternal markers and 5 paternal markers.Based on segregation patterns at all markers analyzed,we concluded that none of the progeny analyzed were from self-fertilization,and one-way hybridization between two individuals was successful in both of the two bay scallop families. |
| 文献子类 | CNKI期刊论文 |
| 资助机构 | 山东省科技发展计划项目(022110107) ; 青岛市科技计划项目(03-1-HH-10) ; 中国科学院知识创新工程重要方向课题(ZKCX2-211) ; 国家杰出青年基金(39825121) |
| 期 | 05页:139-145 |
| 语种 | 中文; |
| 分类号 | S917.4 |
| ISSN号 | 1000-0615 |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/198495]  |
| 专题 | 中国科学院海洋研究所 |
| 作者单位 | 1.NJ08349USA 2.山东青岛266071 3.美国新泽西州立大学Haskin贝类研究实验室山东青岛266071 4.中国科学院海洋研究所 |
| 推荐引用方式 GB/T 7714 | 孙博,刘晓,张国范,等. 海湾扇贝个体间单向人工授精的分子生物学验证. 2006. |
入库方式： OAI收割
来源：海洋研究所
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