A novel enzyme-assisted one-pot method for the extraction of fucoidan and alginate oligosaccharides from Lessonia trabeculata and their bioactivities
文献类型：期刊论文
| 作者 | Yan, Changhui2,4; Pan, Mingxuan3; Geng, Lihua1,4; Zhang, Quanbin1,4; Hu, Yadong3; Wang, Jing1,4 ; Ye, Sujuan2
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| 刊名 | JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| 出版日期 | 2024-08-12 |
| 页码 | 15 |
| 关键词 | Lessonia trabeculata alkaline digestion-enzymatic hydrolysis alginate oligosaccharides fucoidan antioxidant activity |
| ISSN号 | 2096-5508 |
| DOI | 10.1007/s00343-024-4042-8 |
| 通讯作者 | Hu, Yadong(huyadong@jsyhkf.com) ; Wang, Jing(jingwang@qdio.ac.cn) |
| 英文摘要 | Alginate oligosaccharides (AOS) and fucoidan are two important bioactive carbohydrate which that can be prepared from brown seaweeds. We investigated a novel enzyme-assisted one-pot method on AOS and fucoidan extraction from Lessonia trabeculata, and found that the optimum extraction procedure was: first, using 10-g/L Na2CO3 solution to digest the seaweeds for 2 h at 80 degrees C with the material-liquid ratio 1.20 (g/mL), and then adding 4.0% fucoidan cleavage enzyme for reaction for 12 h at 45 degrees C. The yield of AOS and fucoidan was 21.36% and 14.40%, respectively, which was higher than those using traditional methods. The degree of polymerization of AOS was determined to be 3-5, confirming the results through thin layer chromatography (TLC) and electrospray ionization mass spectrometry (ESI-MS). The ratio of guluronic acid to mannuronic acid in AOS was found to be 1.97. Through anion-exchange column chromatography, four sulfated polysaccharide fractions (F0.5, F1.0, F1.5, and F2.0) were successfully isolated from fucoidan. Chemical analysis revealed that fucoidan and F0.5 were heteropolysaccharides, consisting mainly of mannuronic acid, fucose, and xylose, while the other fractions were predominantly composed of fucose and galactose. The sulfate group content increased from 8.74% to 18.76%, while the uronic acid content decreased from 23.79% to 5.58% across the fractions. All fractions of fucoidan exhibited significant antioxidant activity in various tests. There was a positive correlation between the sulfate content and the ability to scavenge superoxide radicals. Additionally, both AOS and the fucoidan fractions showed immunostimulation activity and were nontoxic to RAW264.7 cells. These results indicate that the novel enzyme-assisted one-pot method is a green and efficient approach for the extraction of fucoidan and alginate oligosaccharides from brown seaweeds. It has potential applications in the processing industry of brown seaweeds. |
| WOS关键词 | SACCHARINA-JAPONICA ; SODIUM ALGINATE ; POLYSACCHARIDES ; ACID ; DEGRADATION ; SUGARS ; ALGAE ; LYASE |
| 资助项目 | Jiangsu Coast Development Group Co., Ltd. ; Marine Biological High Value Development and Utilization Project[2023YHTZZZ04] ; Natural Science Foundation of Shandong Province[ZR2022MD007] ; Science and Technology Project of Fujian Province[2022T3024] ; Science and Technology Project of Fujian Province[2023T3057] |
| WOS研究方向 | Marine & Freshwater Biology ; Oceanography |
| 语种 | 英语 |
| WOS记录号 | WOS:001289957200001 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/196530]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Hu, Yadong; Wang, Jing |
| 作者单位 | 1.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 2.Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Qingdao 266061, Peoples R China 3.Jiangsu Coast Dev Investment Co Ltd, Jiangsu Coast Dev Grp Co Ltd, Jiangsu Innovat Ctr Marine Bioresource, Nanjing 210019, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Megasci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan, Changhui,Pan, Mingxuan,Geng, Lihua,et al. A novel enzyme-assisted one-pot method for the extraction of fucoidan and alginate oligosaccharides from Lessonia trabeculata and their bioactivities[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2024:15. |
| APA | Yan, Changhui.,Pan, Mingxuan.,Geng, Lihua.,Zhang, Quanbin.,Hu, Yadong.,...&Ye, Sujuan.(2024).A novel enzyme-assisted one-pot method for the extraction of fucoidan and alginate oligosaccharides from Lessonia trabeculata and their bioactivities.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,15. |
| MLA | Yan, Changhui,et al."A novel enzyme-assisted one-pot method for the extraction of fucoidan and alginate oligosaccharides from Lessonia trabeculata and their bioactivities".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2024):15. |
入库方式： OAI收割
来源：海洋研究所
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