Response surface optimization of preparation parameters and evaluation of the antioxidant and antihypertensive activities of Spirulina protein hydrolysates
文献类型：期刊论文
| 作者 | Suo, Qishan1,2,3,4 ; Deng, Zhenzhen1,2,3,4; Yue, Yang1,3,4; Wang, Jing1,3,4; Wu, Ning1,3,4; Geng, Lihua1,3,4; Zhang, Quanbin1,2,3,4
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| 刊名 | JOURNAL OF OCEANOLOGY AND LIMNOLOGY
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| 出版日期 | 2024-09-02 |
| 页码 | 14 |
| 关键词 | antioxidant activity antihypertensive effect angiotensin converting enzyme inhibition response surface methodology Spirulina |
| ISSN号 | 2096-5508 |
| DOI | 10.1007/s00343-024-3258-y |
| 通讯作者 | Yue, Yang(yueyang@qdio.ac.cn) |
| 英文摘要 | Safety, high protein content, and the numerous health benefits make Spirulina an outstanding source of bioactive peptides. Peptides from Spirulina, which exhibit antioxidant, anti-inflammatory, and antihypertensive effects, may assist in the management of cardiovascular diseases (CVDs). Here, we present the optimization of enzyme-digested hydrolysates derived from Spirulina sp. by using response surface methodology (RSM). We also evaluated the biological activity of Spirulina protein hydrolysates (SPHs) in contributing to the amelioration of hypertension and associated cardiovascular diseases. Our findings suggest that Spirulina protein extracts contain a complex proteinaceous composition with phycocyanin being the dominant protein, as evidenced by both SDS-PAGE and LC-MS/MS profiling. Alkaline protease, protease K, and alpha-chymotrypsin exhibited a substantial hydrolytic effect on Spirulina protein, taking into account their degree of hydrolysis (DH), peptide yield, and molecular weight distribution. Single-factor experiments identified pH, temperature, and enzyme/substrate ratio as the key factors influencing DH. Moreover, optimal hydrolysis conditions for the proteases were determined via RSM, leading to a considerable enhancement of the actual DH. Bioassays showed that SPHs have strong antioxidant activity against various free radicals, with a higher ability to scavenge hydroxyl radicals. Furthermore, SPHs inhibited macrophage nitric oxide secretion and significantly inhibited angiotensin I-converting enzyme in vitro at 400 mu g/mL. It is worth noting that SPHs prepared with protease K exhibited a potent anti-hypertensive effect in vivo. The findings indicate the potential usefulness of including dietary SPHs in preventing hypertension and associated CVDs. This research could be valuable in guiding the development of health-promoting foods within the Spirulina industry. |
| WOS关键词 | ARTHROSPIRA-PLATENSIS ; BIOACTIVE PEPTIDES ; OXIDATIVE STRESS ; IDENTIFICATION ; HYPERTENSION ; PHYCOCYANIN ; DISEASE ; CANCER |
| 资助项目 | Science and Technology Project of Fujian Province[2023T3034] ; Science and Technology Project of Fujian Province[2020N3011] ; Innovation Academy for Drug Discovery and Development, Chinese Academy of Sciences |
| WOS研究方向 | Marine & Freshwater Biology ; Oceanography |
| 语种 | 英语 |
| WOS记录号 | WOS:001303907800003 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/198206]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Yue, Yang |
| 作者单位 | 1.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 2.Univ Chinese Acad Sci, Beijing 101408, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Suo, Qishan,Deng, Zhenzhen,Yue, Yang,et al. Response surface optimization of preparation parameters and evaluation of the antioxidant and antihypertensive activities of Spirulina protein hydrolysates[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2024:14. |
| APA | Suo, Qishan.,Deng, Zhenzhen.,Yue, Yang.,Wang, Jing.,Wu, Ning.,...&Zhang, Quanbin.(2024).Response surface optimization of preparation parameters and evaluation of the antioxidant and antihypertensive activities of Spirulina protein hydrolysates.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,14. |
| MLA | Suo, Qishan,et al."Response surface optimization of preparation parameters and evaluation of the antioxidant and antihypertensive activities of Spirulina protein hydrolysates".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2024):14. |
入库方式： OAI收割
来源：海洋研究所
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