CRADD and cIAP1 antagonistically regulate caspase-9-mediated apoptosis in teleost
文献类型：期刊论文
| 作者 | Wu, Meng1,2,3,4; Chen, Yuan1,2,3,4; Yuan, Zihao1,2,3,4 ; Xu, Hang2,3,4; Sun, Li1,2,3,4
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| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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| 出版日期 | 2024-11-01 |
| 卷号 | 279页码:9 |
| 关键词 | Caspase cIAP CRADD Apoptosis Regulation |
| ISSN号 | 0141-8130 |
| DOI | 10.1016/j.ijbiomac.2024.135265 |
| 通讯作者 | Xu, Hang(xuhang@qdio.ac.cn) ; Sun, Li(lsun@qdio.ac.cn) |
| 英文摘要 | Caspase 9 (CASP9) is a well-known initiator caspase of intrinsic apoptosis. In humans, cIAP1 binds and induces degradation of the activated form of CASP9, but not pro-CASP9. In fish, the activity and regulation of CASP9 remain unknown. In this work, using flounder Paralichthys olivaceus as a representative species, we examined the regulatory mechanism of CASP9 in teleost. P. olivaceus CASP9 (PoCASP9) induced robust apoptosis, which was inhibited by P. olivaceus cIAP1 (PocIAP1). Unlike human cIAP1, PocIAP1 bound both proand active PoCASP9 and induced their degradation via the RING domain-involved proteasome pathway. In humans, the adaptor molecule CRADD cannot interact with CASP9. In contrast, P. olivaceus CRADD (PoCRADD) bound both proand active PoCASP9 via CARD-CARD interaction and enhanced apoptosis by promoting the cellular levels of proand active PoCASP9. Furthermore, PoCRADD abrogated the inhibition of PoCASP9 by PocIAP1 by preventing PocIAP1-PoCASP9 interaction. Together these results reveal a CASP9 regulation mechanism in teleost that differs from that in humans and demonstrate that teleost CASP9 is tightly and directly controlled by both negative and positive regulators that exert a regulation effect both before and after CASP9 activation. These findings advance our understanding of the regulation of CASP9-mediated apoptosis in vertebrates. |
| WOS关键词 | MOLECULAR-CLONING ; CYTOCHROME-C ; CELL-DEATH ; ACTIVATION ; MECHANISMS ; BINDING ; RAIDD ; GENE ; XIAP |
| 资助项目 | Science & Technology Innovation Project of Laoshan Laboratory[LSKJ202203000] ; National Natural Science Foundation of China[31730100] ; Postdoctoral Fellowship Program of China Postdoctoral Science Foundation[GZC20232709] ; National Key Research and Development Program of China[2018YFD0900500] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:001309080800001 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/198653]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Xu, Hang; Sun, Li |
| 作者单位 | 1.Univ Chinese Acad Sci, Coll Marine Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, CAS, Qingdao, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 4.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Meng,Chen, Yuan,Yuan, Zihao,et al. CRADD and cIAP1 antagonistically regulate caspase-9-mediated apoptosis in teleost[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2024,279:9. |
| APA | Wu, Meng,Chen, Yuan,Yuan, Zihao,Xu, Hang,&Sun, Li.(2024).CRADD and cIAP1 antagonistically regulate caspase-9-mediated apoptosis in teleost.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,279,9. |
| MLA | Wu, Meng,et al."CRADD and cIAP1 antagonistically regulate caspase-9-mediated apoptosis in teleost".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 279(2024):9. |
入库方式： OAI收割
来源：海洋研究所
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