Identification of candidate genes and pathways involved in the establishment of sexual size dimorphism in the olive flounder (Paralichthys olivaceus) using RNA-seq
文献类型：期刊论文
| 作者 | Zhang, Yuqing2; Zhang, Wenying2; Jian, Yanmin2; Zhang, Shujing2; Liu, Dongwu2; Zheng, Kang2; Tan, Xungang1,3,4 ; You, Feng1,3,4; Pang, Qiuxiang2; Jiao, Shuang2
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| 刊名 | AQUACULTURE
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| 出版日期 | 2025-01-30 |
| 卷号 | 595页码:12 |
| 关键词 | Sex-dependent growth differences Gene expression SSD developmental window Reproductive axis Somatotropic endocrine axis |
| ISSN号 | 0044-8486 |
| DOI | 10.1016/j.aquaculture.2024.741604 |
| 通讯作者 | Pang, Qiuxiang(pangqiuxiang@sdut.edu.cn) ; Jiao, Shuang(jiaoshuang@sdut.edu.cn) |
| 英文摘要 | Olive flounder (Paralichthys olivaceus) is a valuable mariculture fish that shows female-biased sexual size dimorphism (SSD), yet the molecular mechanisms responsible for this phenomenon remain poorly understood. In this study, the growth differences between females and males were compared, and comparative transcriptome analyses of brains/pituitaries, livers, muscles, and gonads from 13-month-old females and males were performed. The critical time window for SSD was from 13- to 14-month-old, with females reaching significantly larger sizes than males. Muscle cell size and plasma growth hormone (GH), insulin-like growth factor 1 and 2 (IGF1, IGF2), 11-ketotestosterone (11-KT), and 17 beta-estradiol (E2) levels had no significant differences between the sexes of 13and 14-month-old flounders. Furthermore, the RNA-seq data revealed differential expression in the genes encoding the receptors for these hormones, suggesting that regulation may occur at the receptor level, potentially leading to SSD during the critical window. RNA-seq analysis identified 88, 645, 414, and 15,833 differentially expressed genes between females and males in brains/pituitaries, livers, muscles, and gonads, respectively. KEGG (Kyoto Encyclopedia of Genes and Genomes) and GSEA-based KEGG analyses revealed that some pathways associated with growth and development, including "prolactin signaling pathway" in brains/pituitaries, "cell cycle", "oocyte meiosis", and "DNA replication" in livers, "focal adhesion", "ECM-receptor interaction", "protein digestion and absorption", "arginine and proline metabolism", and "cysteine and methionine metabolism" in muscles, and "Hippo signaling pathway" and "JAK-STAT signaling pathway" in gonads may be involved in the establishment of sexual growth differences. Notably, females had lower steroid hormone synthesis abilities than males in brains/pituitaries, livers, and gonads, indicating the importance of sex steroid hormones in the establishment of SSD. Moreover, genes including frizzled3 (fzd3) and prolactin (prl) in brains/pituitaries, and growth hormone receptor (ghr), estrogen receptor (esr1), insulin-like growth factor binding protein-3 (igfbp3), bone morphogenetic protein 8A-like (bmp8a), leptin receptor (lepr), and lipoprotein lipase-like (lpl) in livers emerge as potential candidates for the establishment of SSD. In conclusion, this study may help clarify the molecular mechanisms involved in SSD-related growth traits in flounder and provide basic data for genetic breeding of flounder. |
| WOS关键词 | GROWTH-FACTOR-I ; EXPRESSION ANALYSIS ; YELLOW CATFISH ; FEMALE ; FISH ; AXIS ; MANIPULATION ; STEROIDS ; MARKERS ; TILAPIA |
| 资助项目 | National Natural Science Foundation of China[32302975] ; Key Research and Development Program of Shandong Province, China[2019GHY112007] ; Natural Science Foundation of Shandong Province[ZR2023QC068] |
| WOS研究方向 | Fisheries ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001316350500001 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/198955]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Pang, Qiuxiang; Jiao, Shuang |
| 作者单位 | 1.Chinese Acad Sci, Qingdao 266071, Peoples R China 2.Shandong Univ Technol, Sch Life Sci & Med, Zibo 255000, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Yuqing,Zhang, Wenying,Jian, Yanmin,et al. Identification of candidate genes and pathways involved in the establishment of sexual size dimorphism in the olive flounder (Paralichthys olivaceus) using RNA-seq[J]. AQUACULTURE,2025,595:12. |
| APA | Zhang, Yuqing.,Zhang, Wenying.,Jian, Yanmin.,Zhang, Shujing.,Liu, Dongwu.,...&Jiao, Shuang.(2025).Identification of candidate genes and pathways involved in the establishment of sexual size dimorphism in the olive flounder (Paralichthys olivaceus) using RNA-seq.AQUACULTURE,595,12. |
| MLA | Zhang, Yuqing,et al."Identification of candidate genes and pathways involved in the establishment of sexual size dimorphism in the olive flounder (Paralichthys olivaceus) using RNA-seq".AQUACULTURE 595(2025):12. |
入库方式： OAI收割
来源：海洋研究所
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