Transcriptomic and Biochemical Analysis of the Antimicrobial Mechanism of Lipopeptide Iturin W against Staphylococcus aureus
文献类型：期刊论文
| 作者 | Ji, Yingyu2; Sun, Chaomin1,3,4; Wu, Shimei2 |
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 出版日期 | 2024-09-01 |
| 卷号 | 25期号:18页码:17 |
| 关键词 | Staphylococcus aureus lipopeptide antimicrobial virulence |
| ISSN号 | 1661-6596 |
| DOI | 10.3390/ijms25189949 |
| 通讯作者 | Wu, Shimei(wushimei@qdu.edu.cn) |
| 英文摘要 | Staphylococcus aureus is one of the most serious pathogens threatening food safety and public health. We have previously showed that iturin W exhibited obvious antifungal activity on plant pathogens. In the present study, we found iturin W, especially C-14 iturin W, showed strong antimicrobial activity against S. aureus, and the antimicrobial mechanism of C-14 iturin W was further investigated by transcriptomic analysis and a related biochemical experiment. The results showed that C-14 iturin W can reduce the expression levels of genes associated with the reactive oxygen species (ROS) scavenging enzyme and genes involved in arginine biosynthesis, thus leading to the increase in ROS levels of S. aureus. Furthermore, C-14 iturin W can also interfere with proton dynamics, which is crucial for cells to regulate various biological possesses. Therefore, ROS accumulation and change in proton motive force are import ways for C-14 iturin W to exert the antimicrobial activity. In addition, C-14 iturin W can also reduce the expression levels of genes related to virulence factors and decrease the production of enterotoxins and hemolysins in S. aureus, indicating that C-14 iturin W has a good potential in food and pharmaceutical fields to reduce the harm caused by S. aureus in the future. |
| WOS关键词 | ARGININE ; GENE |
| 资助项目 | Shandong Provincial Natural Science Foundation ; National Natural Science Foundation of China[31600035] ; Science and Technology Innovation Project of Laoshan Laboratory[2022QNLM030004-3] ; Science and Technology Innovation Project of Laoshan Laboratory[LSKJ202203103] ; NSFC Innovative Group Grant[42221005] ; Key Collaborative Research Program of the Alliance of International Science Organizations ; Major Research Plan of the National Natural Science Foundation[92351301] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA22050301] ; Taishan Scholars Program[tstp20230637] ; [ZR2021MD099] ; [ZR2021ZD28] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001326064400001 |
| 出版者 | MDPI |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/199264]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Wu, Shimei |
| 作者单位 | 1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 2.Qingdao Univ, Coll Life Sci, Qingdao 266071, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ji, Yingyu,Sun, Chaomin,Wu, Shimei. Transcriptomic and Biochemical Analysis of the Antimicrobial Mechanism of Lipopeptide Iturin W against Staphylococcus aureus[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2024,25(18):17. |
| APA | Ji, Yingyu,Sun, Chaomin,&Wu, Shimei.(2024).Transcriptomic and Biochemical Analysis of the Antimicrobial Mechanism of Lipopeptide Iturin W against Staphylococcus aureus.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,25(18),17. |
| MLA | Ji, Yingyu,et al."Transcriptomic and Biochemical Analysis of the Antimicrobial Mechanism of Lipopeptide Iturin W against Staphylococcus aureus".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 25.18(2024):17. |
入库方式： OAI收割
来源：海洋研究所
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