The marine environmental microbiome mediates physiological outcomes in host nematodes
文献类型：期刊论文
| 作者 | Xue, Yiming1,2,3,4,5; Xie, Yusu1,2,4,5; Cao, Xuwen1,2,3,4,5 ; Zhang, Liusuo1,2,4,5
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| 刊名 | BMC BIOLOGY
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| 出版日期 | 2024-10-08 |
| 卷号 | 22期号:1页码:27 |
| 关键词 | Litoditis marina Full-length 16S rRNA sequencing Marine bacterial collection Host-microbe interactions Metabolic models Gene knockout Gut microbiota |
| DOI | 10.1186/s12915-024-02021-w |
| 通讯作者 | Zhang, Liusuo(lzhang@qdio.ac.cn) |
| 英文摘要 | Background Nematodes are the most abundant metazoans in marine sediments, many of which are bacterivores; however, how habitat bacteria affect physiological outcomes in marine nematodes remains largely unknown. Results Here, we used a Litoditis marina inbred line to assess how native bacteria modulate host nematode physiology. We characterized seasonal dynamic bacterial compositions in L. marina habitats and examined the impacts of 448 habitat bacteria isolates on L. marina development, then focused on HQbiome with 73 native bacteria, of which we generated 72 whole genomes sequences. Unexpectedly, we found that the effects of marine native bacteria on the development of L. marina and its terrestrial relative Caenorhabditis elegans were significantly positively correlated. Next, we reconstructed bacterial metabolic networks and identified several bacterial metabolic pathways positively correlated with L. marina development (e.g., ubiquinol and heme b biosynthesis), while pyridoxal 5'-phosphate biosynthesis pathway was negatively associated. Through single metabolite supplementation, we verified CoQ10, heme b, acetyl-CoA, and acetaldehyde promoted L. marina development, while vitamin B6 attenuated growth. Notably, we found that only four development correlated metabolic pathways were shared between L. marina and C. elegans. Furthermore, we identified two bacterial metabolic pathways correlated with L. marina lifespan, while a distinct one in C. elegans. Strikingly, we found that glycerol supplementation significantly extended L. marina but not C. elegans longevity. Moreover, we comparatively demonstrated the distinct gut microbiota characteristics and their effects on L. marina and C. elegans physiology. Conclusions Given that both bacteria and marine nematodes are dominant taxa in sedimentary ecosystems, the resource presented here will provide novel insights to identify mechanisms underpinning how habitat bacteria affect nematode biology in a more natural context. Our integrative approach will provide a microbe-nematodes framework for microbiome mediated effects on host animal fitness. |
| WOS关键词 | C. ELEGANS ; R-PACKAGE ; LIFE-SPAN ; GENOME ; ANNOTATION ; SYSTEMS ; BACTERIA ; BIOLOGY ; MITOCHONDRIAL ; UBIQUINONE |
| 资助项目 | Key deployment project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences[COMS2019Q16] ; National Natural Science Foundation of China[32170537] ; National Key R and D Program of China[2018YFD0901301] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[2018SDKJ0302-1] ; The Talents from overseas Program, IOCAS of the Chinese Academy of Sciences ; Qingdao Innovation Leadership Program[16-8-3-19-zhc] ; NIH Office of Research Infrastructure Programs[P40 OD010440] |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:001331348200001 |
| 出版者 | BMC |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/199321]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Zhang, Liusuo |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 5.Chinese Acad Sci, Ctr Ocean Mega Sci, 7 Nanhai Rd, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xue, Yiming,Xie, Yusu,Cao, Xuwen,et al. The marine environmental microbiome mediates physiological outcomes in host nematodes[J]. BMC BIOLOGY,2024,22(1):27. |
| APA | Xue, Yiming,Xie, Yusu,Cao, Xuwen,&Zhang, Liusuo.(2024).The marine environmental microbiome mediates physiological outcomes in host nematodes.BMC BIOLOGY,22(1),27. |
| MLA | Xue, Yiming,et al."The marine environmental microbiome mediates physiological outcomes in host nematodes".BMC BIOLOGY 22.1(2024):27. |
入库方式： OAI收割
来源：海洋研究所
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