Efficient biodegradation of poly(butylene adipate-co-terephthalate) in mild temperature by cutinases derived from a marine fungus
文献类型：期刊论文
| 作者 | Fei, Fan1,2,3,4; Su, Zhenjie1,2,3,4; Liu, Rui2,3,4; Gao, Rongrong5 ; Sun, Chaomin1,2,3,4
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| 刊名 | JOURNAL OF HAZARDOUS MATERIALS
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| 出版日期 | 2024-12-05 |
| 卷号 | 480页码:12 |
| 关键词 | PBAT degradation Marine fungus Cutinase Mild temperature Protein engineering |
| ISSN号 | 0304-3894 |
| DOI | 10.1016/j.jhazmat.2024.136008 |
| 通讯作者 | Sun, Chaomin(sunchaomin@qdio.ac.cn) |
| 英文摘要 | Poly(butylene adipate-co-terephthalate) (PBAT) waste gradually accumulates in the environment, posing ecological risks. Enzymatic hydrolysis holds great potential in the end-of-life management of PBAT, but reported enzymes require high reaction temperatures, limiting their practical industrial applications. In this study, we discovered that the marine fungus Alternaria alternata FB1 can efficiently degrade PBAT at 28 degrees C. Two cutinases designated as AaCut4 and AaCut10, were identified and verified as key enzymes responsible for this degradation process. Notably, the recombinant AaCut10 was able to depolymerize 82.14 % PBAT within 24 h and fully decompose it within 48 h at 37 degrees C. Through protein engineering, the yield of terephthalic acid monomer was increased to 96.01 %, highlighting its potential for facilitating PBAT upcycling. Furthermore, based on the investigation of the distribution patterns of PBAT hydrolases, novel degradative agents have been identified within unique ecological niches, leading to the establishment of a comprehensive screening repository of PBAT hydrolases. Overall, our study provides new candidates for enzymatic PBAT recycling with low energy consumption and offers insights into the PBAT degradation manner in ecosystems. |
| WOS关键词 | POLYETHYLENE TEREPHTHALATE ; DEGRADATION ; HYDROLASE ; KNOWLEDGE |
| 资助项目 | Science and Technology Innovation Project of Laoshan Laboratory[2022QNLM030004-3] ; Science and Technology Innovation Project of Laoshan Laboratory[LSKJ202203103] ; Key Collaborative Research Program of the Alliance of International Science Organizations[ANSO-CR-KP-2022-08] ; NSFC Innovative Group Grant[42221005] ; National Natural Science Foundation[92351301] ; Shandong Provincial Natural Science Foundation[ZR2021ZD28] ; Taishan Scholars Program[tstp20230637] |
| WOS研究方向 | Engineering ; Environmental Sciences & Ecology |
| 语种 | 英语 |
| WOS记录号 | WOS:001334301000001 |
| 出版者 | ELSEVIER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/199330]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 海洋研究所_深海极端环境与生命过程研究中心 |
| 通讯作者 | Sun, Chaomin |
| 作者单位 | 1.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 101408, Peoples R China 2.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Ctr Deep Sea Res, Qingdao 266404, Peoples R China 4.Chinese Acad Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266404, Peoples R China 5.Yantai Vocat Coll, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fei, Fan,Su, Zhenjie,Liu, Rui,et al. Efficient biodegradation of poly(butylene adipate-co-terephthalate) in mild temperature by cutinases derived from a marine fungus[J]. JOURNAL OF HAZARDOUS MATERIALS,2024,480:12. |
| APA | Fei, Fan,Su, Zhenjie,Liu, Rui,Gao, Rongrong,&Sun, Chaomin.(2024).Efficient biodegradation of poly(butylene adipate-co-terephthalate) in mild temperature by cutinases derived from a marine fungus.JOURNAL OF HAZARDOUS MATERIALS,480,12. |
| MLA | Fei, Fan,et al."Efficient biodegradation of poly(butylene adipate-co-terephthalate) in mild temperature by cutinases derived from a marine fungus".JOURNAL OF HAZARDOUS MATERIALS 480(2024):12. |
入库方式： OAI收割
来源：海洋研究所
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