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Chitin Translocation Is Functionally Coupled with Synthesis in Chitin Synthase

文献类型：期刊论文

作者Niu, Suhao2,4,5; Qi, Lei1; Zhang, Xiaoyue2,4,5; He, Dongfang1; Li, Pengwei4,5; Wang, Hao4,5; Bi, Yunchen2,3,4,5
刊名INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
出版日期2024-11-01
卷号25期号:21页码:12
关键词chitin synthase membrane translocation processive glycosyltransferase
ISSN号1661-6596
DOI10.3390/ijms252111667
通讯作者Wang, Hao(wanghao@qdio.ac.cn) ; Bi, Yunchen(yunchenbi@qdio.ac.cn)
英文摘要Chitin, an extracellular polysaccharide, is synthesized by membrane-embedded chitin synthase (CHS) utilizing intracellular substrates. The mechanism of the translocation of synthesized chitin across the membrane to extracellular locations remains unresolved. We prove that the chitin synthase from Phytophthora sojae (PsCHS) is a processive glycosyltransferase, which can rapidly produce and tightly bind with the highly polymerized chitin. We further demonstrate that PsCHS is a bifunctional enzyme, which is necessary and sufficient to translocate the synthesized chitin. PsCHS was purified and then reconstituted into proteoliposomes (PLs). The nascent chitin is generated and protected from chitinase degradation unless detergent solubilizes the PLs, showing that PsCHS translocates the newly produced chitin into the lumen of the PLs. We also attempted to resolve the PsCHS structure of the synthesized chitin-bound state, although it was not successful; the obtained high-resolution structure of the UDP/Mn2+-bound state could still assist in describing the characterization of the PsCHS's transmembrane channel. Consistently, we demonstrate that PsCHS is indispensable and capable of translocating chitin in a process that is tightly coupled to chitin synthesis.
WOS关键词MEMBRANE TRANSLOCATION ; LENGTH CONTROL ; MECHANISM ; GLYCOSYLTRANSFERASES ; CLASSIFICATION ; BIOSYNTHESIS ; HYALURONAN ; MODEL
资助项目National Key R&D Program of China ; National Natural Science Foundation of China[42376136] ; Opening fund of State Key Laboratory of Silkworm Genome Biology[SKLSGB-ORP202105] ; Instrument Improvement Funds of Shandong University Public Technology Platform[ts20230204] ; Shandong Provincial Natural Science Foundation[ZR2024ZD32] ; Pre-Research Project of Major Scientific Facilities in Shandong Province[DKXZZ202205] ; [2023YFE0125400]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:001351234100001
出版者MDPI
源URL[http://ir.qdio.ac.cn/handle/337002/199393]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Wang, Hao; Bi, Yunchen
作者单位1.Shandong Univ, Biomed Res Ctr Struct Anal, Jinan 250012, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS, Qingdao 266071, Peoples R China
5.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714		 Niu, Suhao,Qi, Lei,Zhang, Xiaoyue,et al. Chitin Translocation Is Functionally Coupled with Synthesis in Chitin Synthase[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2024,25(21):12.
APA Niu, Suhao.,Qi, Lei.,Zhang, Xiaoyue.,He, Dongfang.,Li, Pengwei.,...&Bi, Yunchen.(2024).Chitin Translocation Is Functionally Coupled with Synthesis in Chitin Synthase.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,25(21),12.
MLA Niu, Suhao,et al."Chitin Translocation Is Functionally Coupled with Synthesis in Chitin Synthase".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 25.21(2024):12.

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来源：海洋研究所

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