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Paralichthys olivaceus GSDME-mediated pyroptosis is regulated by multiple caspases in different manners

文献类型：期刊论文

作者Hao, Kangwei1,2,3,4; Yuan, Liming1,4; Yu, Chao1,4; Xu, Hang1,2,4; Sun, Li1,2,3,4
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2024-12-01
卷号155页码:8
关键词Gasdermin Caspase Paralichthys olivaceus Teleost
ISSN号1050-4648
DOI10.1016/j.fsi.2024.110002
通讯作者Xu, Hang(xuhang@qdio.ac.cn) ; Sun, Li(lsun@qdio.ac.cn)
英文摘要Pyroptosis is a type of programmed cell death mediated by gasdermin (GSDM). GSDM is activated by caspase (CASP), which cleaves GSDM to release the N-terminal (NT) fragment that forms channels in the plasma membrane and leads to cell death. To date, research on pyroptosis in teleost is limited. In this study, we examined the activation and regulation mechanism of pyroptosis in flounder Paralichthys olivaceus. P. olivaceus gasdermin E (PoGSDME) was found to be cleaved by six P. olivaceus caspases (PoCASP1/3a/3b/7/8a/8b). PoCASP1/3a/3b/7 cleaved primarily at 245FEAD248, which generated an NT fragment (NT248) that induced robust pyroptosis. PoCASP8a/8b cleaved both the full length PoGSDME and NT248 at 202IEKD205, thus destroying the biological activity of PoGSDME and NT248. Nine residues crucial for PoGSDME function were identified, of which, F2, L19, and G85 were essential to plasma membrane translocation. During bacterial infection, PoGSDME and PoCASP1 expressions were significantly upregulated in flounder tissues, and PoGSDME, as well as PoCASP1, activation occurred in flounder cells accompanied with the processing cleavage of IL-1(3 and IL-18. Together these results revealed both the activation and the inhibition mechanisms of GSDME-mediated pyroptosis in flounder, and added new insights into the regulation of pyroptosis in fish.
WOS关键词APOPTOSIS ; DEATH
资助项目Chinese Academy of Sciences
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
WOS记录号WOS:001353596500001
出版者ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
源URL[http://ir.qdio.ac.cn/handle/337002/199441]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Xu, Hang; Sun, Li
作者单位1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS, Qingdao, Peoples R China
2.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
3.Univ Chinese Acad Sci, Coll Marine Sci, Qingdao, Peoples R China
4.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China
推荐引用方式
GB/T 7714		 Hao, Kangwei,Yuan, Liming,Yu, Chao,et al. Paralichthys olivaceus GSDME-mediated pyroptosis is regulated by multiple caspases in different manners[J]. FISH & SHELLFISH IMMUNOLOGY,2024,155:8.
APA Hao, Kangwei,Yuan, Liming,Yu, Chao,Xu, Hang,&Sun, Li.(2024).Paralichthys olivaceus GSDME-mediated pyroptosis is regulated by multiple caspases in different manners.FISH & SHELLFISH IMMUNOLOGY,155,8.
MLA Hao, Kangwei,et al."Paralichthys olivaceus GSDME-mediated pyroptosis is regulated by multiple caspases in different manners".FISH & SHELLFISH IMMUNOLOGY 155(2024):8.

入库方式： OAI收割

来源：海洋研究所

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