1H, 13C and 15N resonance assignments of a shark variable new antigen receptor against hyaluronan synthase
文献类型：期刊论文
| 作者 | Liu, Yuxin1,2,3,5; Wang, Hao2,3,5; Chiu, Cookson K. C.4; Wu, Yujie4; Bi, Yunchen2,3,5 |
| 刊名 | BIOMOLECULAR NMR ASSIGNMENTS
 |
| 出版日期 | 2024-08-14 |
| 页码 | 5 |
| 关键词 | Single domain antibody VNAR NMR spectroscopy assignments |
| ISSN号 | 1874-2718 |
| DOI | 10.1007/s12104-024-10190-6 |
| 通讯作者 | Wu, Yujie(wuyj@szbl.ac.cn) ; Bi, Yunchen(yunchenbi@qdio.ac.cn) |
| 英文摘要 | Single domain antibody (sdAb) is only composed of a variable domain of the heavy-chain-only antibody, which is devoid of light chain and naturally occurring in camelids and cartilaginous fishes. Variable New Antigen Receptor (VNAR), a type of single domain antibody present in cartilaginous fishes such as sharks, is the smallest functional antigen-binding fragment found in nature. The unique features, including flexible paratope, high solubility and outstanding stability make VNAR a promising prospect in antibody drug development and structural biology research. However, VNAR's research has lagged behind camelid-derived sdAb, especially in the field of structural research. Here we report the H-1,N-15,C-13 resonance assignments of a VNAR derived from the immune library of Chiloscyllium plagiosum, termed B2-3, which recognizes the hyaluronan synthase. Analysis of the backbone chemical shifts demonstrates that the secondary structure of VNAR is predominately composed of beta-sheets corresponding to around 40% of the B2-3 backbone. The C beta chemical shift values of cysteine residues, combined with mass spectrometry data, clearly shows that B2-3 contains two pairs of disulfide bonds, which is import for protein stability. The assignments will be essential for determining the high resolution solution structure of B2-3 by NMR spectroscopy. |
| WOS关键词 | PROTEIN ; NANOBODIES |
| 资助项目 | National Natural Science Foundation of China[42376136] ; National Natural Science Foundation of China[DKXZZ202205] ; Research on Simulation Technology and Device of Key Processes of Typical Marine Ecological Disasters in the Pre-Research Project of Major Scientific Facilities in Shandong Province |
| WOS研究方向 | Biophysics ; Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:001291593700001 |
| 出版者 | SPRINGER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/199579]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Wu, Yujie; Bi, Yunchen |
| 作者单位 | 1.Qingdao Univ, Coll Life Sci, Qingdao, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Shenzhen Bay Lab, Shenzhen 518055, Peoples R China 5.Chinese Acad Sci, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Yuxin,Wang, Hao,Chiu, Cookson K. C.,et al. 1H, 13C and 15N resonance assignments of a shark variable new antigen receptor against hyaluronan synthase[J]. BIOMOLECULAR NMR ASSIGNMENTS,2024:5. |
| APA | Liu, Yuxin,Wang, Hao,Chiu, Cookson K. C.,Wu, Yujie,&Bi, Yunchen.(2024).1H, 13C and 15N resonance assignments of a shark variable new antigen receptor against hyaluronan synthase.BIOMOLECULAR NMR ASSIGNMENTS,5. |
| MLA | Liu, Yuxin,et al."1H, 13C and 15N resonance assignments of a shark variable new antigen receptor against hyaluronan synthase".BIOMOLECULAR NMR ASSIGNMENTS (2024):5. |
入库方式： OAI收割
来源：海洋研究所
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