Characterization of water microbiota and their relationship with resident oysters during an oyster mortality event
文献类型：期刊论文
| 作者 | Liu, Mingkun1,2,3,4,8; Li, Qingyuan4,6; Xu, Wenwen1,7; Wang, Luping1,4,8; Wu, Fucun1,2,3,4,8; Tan, Lintao1,7; Li, Li1,2,4,5,6,8; Zhang, Guofan1,2,3,4,8 |
| 刊名 | MICROBIOLOGY SPECTRUM
 |
| 出版日期 | 2024-08-20 |
| 页码 | 16 |
| 关键词 | environmental microbiota intestinal microbiota plasticity oyster mortality resident and transient microbes microbial ecology |
| ISSN号 | 2165-0497 |
| DOI | 10.1128/spectrum.02881-23 |
| 通讯作者 | Li, Li(lili@qdio.ac.cn) |
| 英文摘要 | Microorganisms are vital for the health of marine invertebrates, and their assembly is driven by both deterministic and stochastic factors that regulate residents (innate to the host) and transients (from ambient water). However, the role of water microbiota and the significance of deterministic and stochastic processes in aquatic hosts facing mortality threats are largely unknown. This study examines the shifts in water microbiota during an oyster mortality event using amplicon sequencing and compared with those of resident oysters to disentangle the balance of the deterministic and stochastic factors involved. Water temperature and dissolved oxygen significantly shape the microbial community with a distinct monthly pattern, and Cyanobacteria blooms might exacerbate oyster mortality. The comparative analysis of microbial communities in oysters and water revealed that <= 21% of the genera were shared between oysters and water, implying that water microbiota cannot easily transfer into oysters. Furthermore, these shared genera had different functions, with oysters more involved in promoting host digestion and nutrient acquisition and water bacteria enriched more in functions promoting their own growth and survival. These findings illustrate that oysters may possess specific selection or barrier mechanisms that permit a small percentage of transients, controlled by stochastic factors and having a minimal effect on oyster mortality, to enter, whereas the majority of oyster microbiota are residents governed by deterministic factors. Consequently, oysters exhibit some plasticity in their symbiotic microbiota, enabling them to maintain microbial homeostasis and adapt to complex microbial surroundings. This may be a shared mechanism among marine invertebrates for survival in complex marine environments.IMPORTANCEPacific oysters are widely cultured and play vital ecological roles. However, the summer mortality hinders sustainable oyster farming. Untangling causative mechanisms of oyster mortality is a complex task due to the intricate "interactome" involving environmental factors, hosts, and pathogens. Interactions between hosts and microorganisms offer an ideal avenue for investigating the truth. We systematically investigated the microbial community in water and resident oysters during a summer mortality event and proposed that the assembly of oyster microbiota is primarily governed by deterministic processes independent of mortality. Pathogens mainly originate from resident members of the oyster microbiota, with a limited influence from the microbial community in the water. Additionally, environmental degraders, such as Cyanobacteria blooms, cannot be overlooked as a contributing factor of oyster mortality. This study evaluated the weight of deterministic and stochastic factors in microbial assembly during an oyster mortality event and greatly broadened our understanding of the "interactome" through the interaction between oysters and water in microbiota. Pacific oysters are widely cultured and play vital ecological roles. However, the summer mortality hinders sustainable oyster farming. Untangling causative mechanisms of oyster mortality is a complex task due to the intricate "interactome" involving environmental factors, hosts, and pathogens. Interactions between hosts and microorganisms offer an ideal avenue for investigating the truth. We systematically investigated the microbial community in water and resident oysters during a summer mortality event and proposed that the assembly of oyster microbiota is primarily governed by deterministic processes independent of mortality. Pathogens mainly originate from resident members of the oyster microbiota, with a limited influence from the microbial community in the water. Additionally, environmental degraders, such as Cyanobacteria blooms, cannot be overlooked as a contributing factor of oyster mortality. This study evaluated the weight of deterministic and stochastic factors in microbial assembly during an oyster mortality event and greatly broadened our understanding of the "interactome" through the interaction between oysters and water in microbiota. |
| WOS关键词 | GUT MICROBIOTA ; ASSOCIATIONS |
| 资助项目 | National Key R&D Program of China ; Key Research and Development Program of Shandong[ZFJH202309] ; Key Research and Development Program of Shandong[CARS-49] ; [2022YFD2400304] |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:001295176900001 |
| 出版者 | AMER SOC MICROBIOLOGY |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/199603]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Li, Li |
| 作者单位 | 1.Shandong Technol Innovat Ctr Oyster Seed Ind, Qingdao, Peoples R China 2.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Wuhan, Peoples R China 3.Laoshan Lab, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 4.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Natl & Local Joint Engn Key Lab Ecol Mariculture,C, Qingdao, Peoples R China 5.Laoshan Lab, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao, Peoples R China 6.Univ Chinese Acad Sci, Beijing, Peoples R China 7.Rushan Marine Econ & Dev Ctr, Rushan, Peoples R China 8.Southern Marine Sci & Engn Guangdong Lab, Zhanjiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Mingkun,Li, Qingyuan,Xu, Wenwen,et al. Characterization of water microbiota and their relationship with resident oysters during an oyster mortality event[J]. MICROBIOLOGY SPECTRUM,2024:16. |
| APA | Liu, Mingkun.,Li, Qingyuan.,Xu, Wenwen.,Wang, Luping.,Wu, Fucun.,...&Zhang, Guofan.(2024).Characterization of water microbiota and their relationship with resident oysters during an oyster mortality event.MICROBIOLOGY SPECTRUM,16. |
| MLA | Liu, Mingkun,et al."Characterization of water microbiota and their relationship with resident oysters during an oyster mortality event".MICROBIOLOGY SPECTRUM (2024):16. |
入库方式： OAI收割
来源：海洋研究所
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