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Genome-wide investigation of the DMRT gene family sheds new insight into the regulation of sex differentiation in spotted knifejaw (Oplegnathus punctatus) with fusion chromosomes (Y)

文献类型：期刊论文

作者Zhao, Haixia1,2,3,4,5; Xiao, Yongshuang1,3,4,5; Xiao, Zhizhong1,3,4,5; Wu, Yanduo1,2,3,4,5; Ma, Yuting1,3,4,5; Li, Jun1,3,4,5
刊名INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
出版日期2024-02-01
卷号257页码:16
关键词Genome-wide dmrt family Spotted knifejaw Sex differentiation amhr2
ISSN号0141-8130
DOI10.1016/j.ijbiomac.2023.128638
通讯作者Xiao, Yongshuang(yongshuangxiao@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn)
英文摘要The role of the DMRT family in male sex determination and differentiation is significant, but its regulatory role in spotted knifejaw with Y fusion chromosomes remains unclear. Through genome-wide scanning, transcriptome analysis, qPCR, FISH, and RNA interference (RNAi), we investigated the DMRT family and the dmrt1-based sex regulation network. Seven DMRTs were identified (DMRT1/2 (2a,2b)/6, DMRT4/5, DMRT3), and dmrt gene dispersion among chromosomes is possibly driven by three whole-genome duplications. Transcriptome analysis enriched genes were associated with sex regulation and constructed a network associated with dmrt1. qPCR and FISH results showed the expression dimorphism of sex-related genes in dmrt-related regulatory networks. RNAi experiments indicated a distinct sex regulation mode in spotted knifejaw. Dmrt1 knockdown upregulated malerelated genes (sox9a, sox9b, dmrt1, amh, amhr2) and hsd11b2 expression, which is critical for androgen synthesis. Amhr2 is located on the heterozygous chromosome (Y) and is specifically localized in primary spermatocytes, and is extremely upregulated after dmrt1 knockdown which suggested besides the important role of dmrt1 in male differentiation, the amhr2 along with amhr2/amh system, also play important regulatory roles in maintaining high expression of the hsd11b2 and male differentiation. This study aims to further investigate sex regulatory mechanisms in species with fusion chromosomes.
WOS关键词EXPRESSION ; FISH ; ROLES ; DIVERSITY ; CELLS ; SOX9
资助项目Key Deployment jects of Center for Ocean Mega-Science, Chinese Academy of Sciences[COMS2020Q05] ; Key Deployment jects of Center for Ocean Mega-Science, Chinese Academy of Sciences[CARS-47] ; National Key Research and Development Program[2022YFC3103600]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种英语
WOS记录号WOS:001139548300001
出版者ELSEVIER
源URL[http://ir.qdio.ac.cn/handle/337002/199660]  
专题海洋研究所_实验海洋生物学重点实验室
通讯作者Xiao, Yongshuang; Li, Jun
作者单位1.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Chinese Acad Sci, Qingdao, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
5.Chinese Acad Sci, Key Lab Breeding Biotechnol & Sustainable Aquacult, Wuhan, Peoples R China
推荐引用方式
GB/T 7714		 Zhao, Haixia,Xiao, Yongshuang,Xiao, Zhizhong,et al. Genome-wide investigation of the DMRT gene family sheds new insight into the regulation of sex differentiation in spotted knifejaw (Oplegnathus punctatus) with fusion chromosomes (Y)[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2024,257:16.
APA Zhao, Haixia,Xiao, Yongshuang,Xiao, Zhizhong,Wu, Yanduo,Ma, Yuting,&Li, Jun.(2024).Genome-wide investigation of the DMRT gene family sheds new insight into the regulation of sex differentiation in spotted knifejaw (Oplegnathus punctatus) with fusion chromosomes (Y).INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,257,16.
MLA Zhao, Haixia,et al."Genome-wide investigation of the DMRT gene family sheds new insight into the regulation of sex differentiation in spotted knifejaw (Oplegnathus punctatus) with fusion chromosomes (Y)".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 257(2024):16.

入库方式： OAI收割

来源：海洋研究所

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