Linking morphology, genome, and metabolic activity of uncultured magnetotactic Nitrospirota at the single-cell level
文献类型：期刊论文
| 作者 | Ji, Runjia1,4,5; Wan, Juan1,5; Liu, Jia1,3; Zheng, Jinbo2,4,5; Xiao, Tian1,3; Pan, Yongxin1,4,5; Lin, Wei1,4,5 |
| 刊名 | MICROBIOME
 |
| 出版日期 | 2024-08-24 |
| 卷号 | 12期号:1页码:20 |
| 关键词 | Nitrospirota Magnetotactic bacteria Target-specific mini-metagenomics NanoSIMS Ecophysiology |
| ISSN号 | 2049-2618 |
| DOI | 10.1186/s40168-024-01837-6 |
| 通讯作者 | Lin, Wei(weilin@mail.iggcas.ac.cn) |
| 英文摘要 | BackgroundMagnetotactic bacteria (MTB) are a unique group of microorganisms that sense and navigate through the geomagnetic field by biomineralizing magnetic nanoparticles. MTB from the phylum Nitrospirota (previously known as Nitrospirae) thrive in diverse aquatic ecosystems. They are of great interest due to their production of hundreds of magnetite (Fe3O4) magnetosome nanoparticles per cell, which far exceeds that of other MTB. The morphological, phylogenetic, and genomic diversity of Nitrospirota MTB have been extensively studied. However, the metabolism and ecophysiology of Nitrospirota MTB are largely unknown due to the lack of cultivation techniques.MethodsHere, we established a method to link the morphological, genomic, and metabolic investigations of an uncultured Nitrospirota MTB population (named LHC-1) at the single-cell level using nanoscale secondary-ion mass spectrometry (NanoSIMS) in combination with rRNA-based in situ hybridization and target-specific mini-metagenomics.ResultsWe magnetically separated LHC-1 from a freshwater lake and reconstructed the draft genome of LHC-1 using genome-resolved mini-metagenomics. We found that 10 LHC-1 cells were sufficient as a template to obtain a high-quality draft genome. Genomic analysis revealed that LHC-1 has the potential for CO2 fixation and NO3- reduction, which was further characterized at the single-cell level by combining stable-isotope incubations and NanoSIMS analyses over time. Additionally, the NanoSIMS results revealed specific element distributions in LHC-1, and that the heterogeneity of CO2 and NO3- metabolisms among different LHC-1 cells increased with incubation time.ConclusionsTo our knowledge, this study provides the first metabolic measurements of individual Nitrospirota MTB cells to decipher their ecophysiological traits. The procedure constructed in this study provides a promising strategy to simultaneously investigate the morphology, genome, and ecophysiology of uncultured microbes in natural environments.6gfJ7qtcG81szcFZGMmDYdVideo AbstractConclusionsTo our knowledge, this study provides the first metabolic measurements of individual Nitrospirota MTB cells to decipher their ecophysiological traits. The procedure constructed in this study provides a promising strategy to simultaneously investigate the morphology, genome, and ecophysiology of uncultured microbes in natural environments.6gfJ7qtcG81szcFZGMmDYdVideo Abstract |
| WOS关键词 | INDEPENDENT CHARACTERIZATION ; CANDIDATUS MAGNETOBACTERIUM ; MAGNETOSOME FORMATION ; BACTERIA REVEALS ; GENES ; BIOMINERALIZATION ; DENITRIFICATION ; MAGNETITE ; QUALITY ; ECOPHYSIOLOGY |
| 资助项目 | National Natural Science Foundation of China |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:001297026300001 |
| 出版者 | BMC |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/198092]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Lin, Wei |
| 作者单位 | 1.Chinese Acad Sci, France China Joint Lab Evolut & Dev Magnetotact Mu, Beijing 100029, Peoples R China 2.Chinese Acad Sci, Inst Geol & Geophys, Engn Lab Deep Resources Equipment & Technol, Beijing 100029, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 4.Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing 100049, Peoples R China 5.Chinese Acad Sci, Inst Geol & Geophys, Key Lab Earth & Planetary Phys, Beijing 100029, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ji, Runjia,Wan, Juan,Liu, Jia,et al. Linking morphology, genome, and metabolic activity of uncultured magnetotactic Nitrospirota at the single-cell level[J]. MICROBIOME,2024,12(1):20. |
| APA | Ji, Runjia.,Wan, Juan.,Liu, Jia.,Zheng, Jinbo.,Xiao, Tian.,...&Lin, Wei.(2024).Linking morphology, genome, and metabolic activity of uncultured magnetotactic Nitrospirota at the single-cell level.MICROBIOME,12(1),20. |
| MLA | Ji, Runjia,et al."Linking morphology, genome, and metabolic activity of uncultured magnetotactic Nitrospirota at the single-cell level".MICROBIOME 12.1(2024):20. |
入库方式： OAI收割
来源：海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。