Development of Reliable Male-Specific Molecular Markers for Genetic Sex Identification in Sea Cucumber Apostichopus japonicus
文献类型：期刊论文
| 作者 | Liu, Xinghai2; Sun, Lina1; Li, Ziming2; Zhao, Hongyuan1; Yang, Yujia2 |
| 刊名 | MARINE BIOTECHNOLOGY
 |
| 出版日期 | 2024-08-31 |
| 页码 | 12 |
| 关键词 | Apostichopus japonicus Molecular markers Sex-specific markers Genetic sex identification Sea cucumber breeding |
| ISSN号 | 1436-2228 |
| DOI | 10.1007/s10126-024-10364-2 |
| 通讯作者 | Yang, Yujia(yangyujia@ouc.edu.cn) |
| 英文摘要 | Apostichopus japonicus is an important marine aquaculture species in China, with high nutritional and economic value. In A. japonicus, there is no obvious sexual dimorphism in external appearance, and sex differentiation primarily relies on the observation of mature gonads after dissection, which leads to difficulties in sex identification. The confusion in sex identification greatly reduces breeding efficiency in the sea cucumber industry. Therefore, developing a reliable sex-specific marker is crucial. In this study, we identified 586 male-specific sequences through whole-genome assembly and sequence alignment, but did not identify any female-specific sequences, inferring an XY-type sex determination system in sea cucumbers. We developed a set of male-specific molecular markers to establish an accurate, stable, and widely adaptable genetic sex identification technique for A. japonicus. The male-specific molecular markers were validated with 100% accuracy in sea cucumber populations from six different geographical regions in China. In conclusion, this study provides further evidence for the XY-type sex determination system in A. japonicus and establishes an effective genetic sex identification method for multi-geographic populations, which benefits future study on reproductive biology and has significant implications in sea cucumber aquaculture industry. |
| WOS关键词 | PLATYFISH XIPHOPHORUS-MACULATUS ; TRANSPOSONS |
| 资助项目 | National Key Research and Development Program of China |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001303237600001 |
| 出版者 | SPRINGER |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/198200]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Yang, Yujia |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci & Expt Mari, Qingdao 266071, Peoples R China 2.Ocean Univ China, Key Lab Mariculture, Minist Educ, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Xinghai,Sun, Lina,Li, Ziming,et al. Development of Reliable Male-Specific Molecular Markers for Genetic Sex Identification in Sea Cucumber Apostichopus japonicus[J]. MARINE BIOTECHNOLOGY,2024:12. |
| APA | Liu, Xinghai,Sun, Lina,Li, Ziming,Zhao, Hongyuan,&Yang, Yujia.(2024).Development of Reliable Male-Specific Molecular Markers for Genetic Sex Identification in Sea Cucumber Apostichopus japonicus.MARINE BIOTECHNOLOGY,12. |
| MLA | Liu, Xinghai,et al."Development of Reliable Male-Specific Molecular Markers for Genetic Sex Identification in Sea Cucumber Apostichopus japonicus".MARINE BIOTECHNOLOGY (2024):12. |
入库方式： OAI收割
来源：海洋研究所
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