Selection of protocols for phytoplankton pigment analysis: a comparative study
文献类型：期刊论文
| 作者 | Wang, Jinxiu2,3 ; Kong, Fanzhou1,2,3; Niu, Zhuang4; Yu, Rencheng1,2,3
|
| 刊名 | JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2024-09-14 |
| 页码 | 14 |
| 关键词 | pigment fluorometry high-performance liquid chromatography (HPLC) extraction phytoplankton |
| ISSN号 | 2096-5508 |
| DOI | 10.1007/s00343-024-4025-9 |
| 通讯作者 | Kong, Fanzhou(fzkong@qdio.ac.cn) |
| 英文摘要 | Pigments are widely used as indices for estimation of phytoplankton biomass and composition, and many protocols have been developed to analyze pigments in phytoplankton. Different protocols were compared using four solvents (methanol, 95% methanol, dimethylformamide, and 90% acetone) and two instruments (fluorometer and high-performance liquid chromatography (HPLC) coupled with diode array detector). Analysis of chlorophyll a (Chl a) with fluorometer could lead to over- or underestimation due to the interference from its derivatives in all probability. Among the four extractants, 90% acetone had a high recovery for chlorophylls. In contrast, 95% methanol was a poor extractant for chlorophylls due to the degradation of Chl a, especially in diatoms. The 95% methanol, however, had high extraction efficiencies for most diagnostic xanthophylls. Therefore, the selection of pigment analytical protocols should follow the specific purpose of phytoplankton study. In addition to fluorometry, an HPLC method with 90% acetone as extractant shall be a good choice for the analysis of Chl a to estimate phytoplankton biomass, especially for diatom-dominated samples, while an HPLC method with 95% methanol as extractant be more suitable to characterize different taxa in phytoplankton communities. |
| WOS关键词 | PERFORMANCE LIQUID-CHROMATOGRAPHY ; CHLOROPHYLL-A CONCENTRATIONS ; PHOTOSYNTHETIC PIGMENTS ; MARINE-PHYTOPLANKTON ; EXTRACTION METHODS ; ALGAL PIGMENTS ; CAROTENOIDS ; DEGRADATION ; PRODUCTS ; CHEMTAX |
| WOS研究方向 | Marine & Freshwater Biology ; Oceanography |
| 语种 | 英语 |
| WOS记录号 | WOS:001313441000005 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/198633]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Kong, Fanzhou |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 3.Qingdao Marine Sci & Technol Ctr, Lab Marine Ecol & Environm Sci, Qingdao 266214, Peoples R China 4.Marine Monitoring & Forecasting Ctr Zhejiang Prov, Hangzhou 310007, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Jinxiu,Kong, Fanzhou,Niu, Zhuang,et al. Selection of protocols for phytoplankton pigment analysis: a comparative study[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2024:14. |
| APA | Wang, Jinxiu,Kong, Fanzhou,Niu, Zhuang,&Yu, Rencheng.(2024).Selection of protocols for phytoplankton pigment analysis: a comparative study.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,14. |
| MLA | Wang, Jinxiu,et al."Selection of protocols for phytoplankton pigment analysis: a comparative study".JOURNAL OF OCEANOLOGY AND LIMNOLOGY (2024):14. |
入库方式： OAI收割
来源：海洋研究所
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