Intragenomic variations of the harmful algal bloom species Phaeocystis globosa through single-strain metabarcoding analysis
文献类型：期刊论文
| 作者 | Wang, Yifei1,2,3,5; Chen, Nansheng1,2,3,4 |
| 刊名 | MARINE POLLUTION BULLETIN
 |
| 出版日期 | 2024-12-01 |
| 卷号 | 209页码:11 |
| 关键词 | Phaeocystis globosa Metabarcoding analysis 18S rDNA V4 Intragenomic variations |
| ISSN号 | 0025-326X |
| DOI | 10.1016/j.marpolbul.2024.117180 |
| 通讯作者 | Chen, Nansheng(chenn@qdio.ac.cn) |
| 英文摘要 | Recent studies demonstrated that the existence of high-levels intragenomic variations (IGVs) may lead to overinterpretation of species diversity and genetic diversity in metabarcoding analysis. In this study, IGVs of the V4 region of 18S rRNA gene (18S rDNA V4) in Phaeocystis globosa were ascertained through metabarcoding analysis results of 54 P. globosa strains. Each P. globosa strain harbored over 200 ASVs, in which one ASV was dominant with higher relative abundance than others. According to different dominant ASVs, 54 strains could be divided into two groups, suggesting the high genetic diversity of P. globosa. ASVs were shared in different strains, suggesting that IGVs were genuine existed, rather than sequencing errors. Metabarcoding analysis of field samples identified large numbers of IGVs. High levels of IGVs may be due to incomplete homogenization or existence of nonfunctional pseudogenes. An accurate understanding of IGVs is necessary for the correct interpretation of metabarcoding analysis results. |
| WOS关键词 | SEAWATER VISCOSITY ; RIBOSOMAL DNA ; POLYMORPHISM ; NUCLEAR ; PRYMNESIOPHYCEAE ; DINOPHYCEAE ; DIATOM ; PLANT |
| 资助项目 | Natural Science Foundation of China[42176162] ; Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology (Qingdao)[LSKJ202203700] ; Taishan Scholar Project Special Fund |
| WOS研究方向 | Environmental Sciences & Ecology ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001349612200001 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/199402]  |
| 专题 | 海洋研究所_海洋生态与环境科学重点实验室 |
| 通讯作者 | Chen, Nansheng |
| 作者单位 | 1.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 2.Qingdao Marine Sci & Technol Ctr, Lab Marine Ecol & Environm Sci, Qingdao 266200, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, CAS Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Peoples R China 4.Simon Fraser Univ, Dept Mol Biol & Biochem, 8888 Univ Dr, Burnaby, BC V5A 1S6, Canada 5.Univ Chinese Acad Sci, Coll Marine Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yifei,Chen, Nansheng. Intragenomic variations of the harmful algal bloom species Phaeocystis globosa through single-strain metabarcoding analysis[J]. MARINE POLLUTION BULLETIN,2024,209:11. |
| APA | Wang, Yifei,&Chen, Nansheng.(2024).Intragenomic variations of the harmful algal bloom species Phaeocystis globosa through single-strain metabarcoding analysis.MARINE POLLUTION BULLETIN,209,11. |
| MLA | Wang, Yifei,et al."Intragenomic variations of the harmful algal bloom species Phaeocystis globosa through single-strain metabarcoding analysis".MARINE POLLUTION BULLETIN 209(2024):11. |
入库方式： OAI收割
来源：海洋研究所
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