Proteomics and EPS Compositional Analysis Reveals Desulfovibrio bisertensis SY-1 Induced Corrosion on Q235 Steel by Biofilm Formation
文献类型：期刊论文
| 作者 | Wang, Yanan2,3 ; Zhang, Ruiyong1,3; Mathivanan, Krishnamurthy3; Zhang, Yimeng3; Yang, Luhua3; Guan, Fang1,3; Duan, Jizhou1,3
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| 刊名 | MATERIALS
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| 出版日期 | 2024-10-01 |
| 卷号 | 17期号:20页码:20 |
| 关键词 | planktonic cells biofilm formation microbially influenced corrosion extracellular polymeric substances proteomics |
| DOI | 10.3390/ma17205060 |
| 通讯作者 | Zhang, Ruiyong(ruiyong.zhang@qdio.ac.cn) ; Duan, Jizhou(duanjz@qdio.ac.cn) |
| 英文摘要 | Microorganisms that exist in the seawater form microbial biofilms on materials used in marine construction, especially on metal surfaces submerged in seawater, where they form biofilms and cause severe corrosion. Biofilms are mainly composed of bacteria and their secreted polymeric substances. In order to understand how biofilms promote metal corrosion, planktonic and biofilm cells of Desulfovibrio bizertensis SY-1 (D. bizertensis) from Q235 steel were collected and analyzed as to their intracellular proteome and extracellular polymeric substances (EPS). The intracellular proteome analysis showed that the cellular proteins were strongly regulated in biofilm cells compared to planktonic cells, e.g., along with flagellar proteins, signaling-related proteins were significantly increased, whereas energy production and conversion proteins and DNA replication proteins were significantly regulated. The up-and-down regulation of proteins revealed that biofilm formation by bacteria on metal surfaces is affected by flagellar and signaling proteins. A significant decrease in DNA replication proteins indicated that DNA is no longer replicated and transcribed in mature biofilms, thus reducing energy consumption. Quantitative analysis and lectin staining of the biofilm on the metal's surface revealed that the bacteria secreted a substantial amount of EPS when they began to attach to the surface, and proteins dominated the main components of EPS. Further, the infrared analysis showed that the secondary structure of the proteins in the EPS of the biofilm was mainly dominated by beta-sheet and 3-turn helix, which may help to enhance the adhesion of EPS. The functional groups of EPS analyzed using XPS showed that the C element of EPS in the biofilm mainly existed in the form of combinations with N. Furthermore, the hydroxyl structure in the EPS extracted from the biofilm had a stronger hydrogen bonding effect, which could maintain the stability of the EPS structure and biofilm. The study results revealed that D. bizertensis regulates the metabolic pathways and their secreted EPS structure to affect biofilm formation and cause metal corrosion, which has a certain reference significance for the study of the microbially influenced corrosion (MIC) mechanism. |
| WOS关键词 | SULFATE-REDUCING BACTERIA ; EXTRACELLULAR POLYMERIC SUBSTANCES ; SAMPLE PREPARATION METHOD ; PROTEOGENOMIC CHARACTERIZATION ; ENERGY-CONSERVATION ; THIAMINE SYNTHESIS ; ELECTRON-TRANSFER ; ESCHERICHIA-COLI ; CARBON-STEEL ; IRON |
| 资助项目 | National Natural Science Foundation of China ; Key Research Program of Frontier Sciences, CAS[ZDBS-LY-DQC025] ; [42076044] |
| WOS研究方向 | Chemistry ; Materials Science ; Metallurgy & Metallurgical Engineering ; Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:001341667100001 |
| 出版者 | MDPI |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/199570]  |
| 专题 | 海洋研究所_海洋腐蚀与防护研究发展中心 |
| 通讯作者 | Zhang, Ruiyong; Duan, Jizhou |
| 作者单位 | 1.Guangxi Acad Sci, Inst Marine Corros Protect, Guangxi Key Lab Marine Environm Sci, Nanning 530007, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Key Lab Adv Marine Mat, Key Lab Marine Environm Corros & Biofouling, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yanan,Zhang, Ruiyong,Mathivanan, Krishnamurthy,et al. Proteomics and EPS Compositional Analysis Reveals Desulfovibrio bisertensis SY-1 Induced Corrosion on Q235 Steel by Biofilm Formation[J]. MATERIALS,2024,17(20):20. |
| APA | Wang, Yanan.,Zhang, Ruiyong.,Mathivanan, Krishnamurthy.,Zhang, Yimeng.,Yang, Luhua.,...&Duan, Jizhou.(2024).Proteomics and EPS Compositional Analysis Reveals Desulfovibrio bisertensis SY-1 Induced Corrosion on Q235 Steel by Biofilm Formation.MATERIALS,17(20),20. |
| MLA | Wang, Yanan,et al."Proteomics and EPS Compositional Analysis Reveals Desulfovibrio bisertensis SY-1 Induced Corrosion on Q235 Steel by Biofilm Formation".MATERIALS 17.20(2024):20. |
入库方式： OAI收割
来源：海洋研究所
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