Deciphering deep-sea chemosynthetic symbiosis by single-nucleus RNA-sequencing
文献类型：期刊论文
| 作者 | Wang, Hao5,6,7,8; He, Kai4; Zhang, Huan8; Zhang, Quanyong3; Cao, Lei8 ; Li, Jing2; Zhong, Zhaoshan8; Chen, Hao8; Zhou, Li8; Lian, Chao8
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| 刊名 | ELIFE
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| 出版日期 | 2024-08-05 |
| 卷号 | 12页码:26 |
| 关键词 | cold-seep chemosynthetic endosymbiosis snRNA-seq cell atlas Gigantidas platifrons transplantation Other |
| ISSN号 | 2050-084X |
| DOI | 10.7554/eLife.88294 |
| 通讯作者 | Wang, Hao(haowang@qdio.ac.cn) ; Qian, Pei-Yuan(boqianpy@ust.hk) ; Li, Chaolun(lcl@qdio.ac.cn) |
| 英文摘要 | Bathymodioline mussels dominate deep-sea methane seep and hydrothermal vent habitats and obtain nutrients and energy primarily through chemosynthetic endosymbiotic bacteria in the bacteriocytes of their gill. However, the molecular mechanisms that orchestrate mussel host-symbiont interactions remain unclear. Here, we constructed a comprehensive cell atlas of the gill in the mussel Gigantidas platifrons from the South China Sea methane seeps (1100 m depth) using single-nucleus RNA-sequencing (snRNA-seq) and whole-mount in situ hybridisation. We identified 13 types of cells, including three previously unknown ones, and uncovered unknown tissue heterogeneity. Every cell type has a designated function in supporting the gill's structure and function, creating an optimal environment for chemosynthesis, and effectively acquiring nutrients from the endosymbiotic bacteria. Analysis of snRNA-seq of in situ transplanted mussels clearly showed the shifts in cell state in response to environmental oscillations. Our findings provide insight into the principles of host-symbiont interaction and the bivalves' environmental adaption mechanisms. |
| WOS关键词 | BATHYMODIOLUS MUSSELS ; PARTICLE-TRANSPORT ; COLD SEEP ; GILL ; EXPRESSION ; ENDOSYMBIONTS ; METHANE ; CARBON ; TRANSCRIPTOME ; ENVIRONMENTS |
| 资助项目 | Science and Technology Innovation Project of Laoshan Laboratory ; National Natural Science Foundation of China[42030407] ; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)[HJ202101] ; Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou)[SMSEGL24SC01] ; Major Project of Basic and Applied Basic Research of Guangdong Province[2019B030302004] ; Research Grants Council of Hong Kong[C2013-22G] ; Research Grants Council of Hong Kong[16101822] ; Guangdong Natural Science Funds for Distinguished Young Scholar[2022B1515020033] ; [LSKJ202203104] |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:001284940900001 |
| 出版者 | eLIFE SCIENCES PUBL LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/197677]  |
| 专题 | 海洋研究所_深海极端环境与生命过程研究中心 |
| 通讯作者 | Wang, Hao; Qian, Pei-Yuan; Li, Chaolun |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, South China Sea Inst Oceanol, Guangzhou, Peoples R China 3.Kunming Univ Sci & Technol, Inst Primate Translat Med, State Key Lab Primate Biomed Res, Kunming, Peoples R China 4.Guangzhou Univ, Sch Life Sci, Key Lab Conservat & Applicat Biodivers South China, Guangzhou, Peoples R China 5.Hong Kong Univ Sci & Technol, Dept Ocean Sci, Hong Kong, Peoples R China 6.Southern Marine Sci & Engn, Guangdong Lab Guangzhou, Guangzhou, Peoples R China 7.Qingdao Marine Sci & Technol Ctr, Laoshan Lab, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 8.Chinese Acad Sci, Ctr Deep Sea Res, Inst Oceanol, Qingdao, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Hao,He, Kai,Zhang, Huan,et al. Deciphering deep-sea chemosynthetic symbiosis by single-nucleus RNA-sequencing[J]. ELIFE,2024,12:26. |
| APA | Wang, Hao.,He, Kai.,Zhang, Huan.,Zhang, Quanyong.,Cao, Lei.,...&Li, Chaolun.(2024).Deciphering deep-sea chemosynthetic symbiosis by single-nucleus RNA-sequencing.ELIFE,12,26. |
| MLA | Wang, Hao,et al."Deciphering deep-sea chemosynthetic symbiosis by single-nucleus RNA-sequencing".ELIFE 12(2024):26. |
入库方式： OAI收割
来源：海洋研究所
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