Structural elucidation of an active arabinoglucan from Gomphrena globosa and its protection effect and mechanism against metabolic dysfunction-associated steatohepatitis
文献类型：期刊论文
| 作者 | Chen, Meilin4,7; Zong, Jianing5; He, Fei4,7; Zhou, Wanqi1,4,6; Liu, Renjie4,7; Xia, Haoran4,5; Mao, Mengfei4,7; Jin, Can2; Wang, Kaiping3; Ding, Kan1,2,4,5,6,7
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| 刊名 | CARBOHYDRATE POLYMERS
 |
| 出版日期 | 2025-01-15 |
| 卷号 | 348页码:15 |
| 关键词 | Arabinoglucan Gomphrena globosa MASH Thioredoxin |
| ISSN号 | 0144-8617 |
| DOI | 10.1016/j.carbpol.2024.122860 |
| 通讯作者 | He, Fei(hefei1@simm.ac.cn) ; Ding, Kan(dingkan@simm.ac.cn) |
| 英文摘要 | The flower of Gomphrena globosa (G. globosa.) is used as Chinese traditional medicine and functional food. Extractions from G. globosa. have been reported to exert hepatoprotective effects. We further hypothesized that the polysaccharide components, as a bioactive ingredient, might have anti-fatty and hepatitis function. Here, a novel homogeneous arabinoglucan GGL0.05S1 (Mw = 83.9 kDa) from this flower, was characterized by monosaccharide composition analysis, methylation analysis, and NMR. Structural analysis showed that the backbone of GGL0.05S1 consists of-*4)-alpha-Glcp-(1-* and-*4,6)-alpha-Glcp-(1-* residues, and the branched chain linked to the main chain via C-6 of-*4,6)-alpha-Glcp-(1-* in the form alpha-Araf-(1-*[4)-alpha-Glcp-(1]b-* (b > 2). In vitro experiments demonstrated that GGL0.05S1 could inhibit lipid deposition and ROS overload in free fatty acid-induced hepatocytes, meanwhile in vivo tests showed that GGL0.05S1 effectively protected against liver injury, steatohepatitis, and fibrosis in a CDA-HFD-fed MASH model. Mechanism study further uncovered that GGL0.05S1 augmented the expression and antioxidant ability of thioredoxin protein that ameliorated oxidative stress, promoted fatty acid beta-oxidation and mitophagy, up to reducing lipotoxicity and alleviating inflammation via inhibiting NLRP3 signaling pathway. Overall, GGL0.05S1 might be a potential novel active compound with liver- protective effect from G. globosa., and which is informative for anti-MASH new drug development. |
| WOS关键词 | FATTY LIVER-DISEASE ; RICE BRAN ; POLYSACCHARIDE ; NASH ; CHROMATOGRAPHY ; CARBAZOLE ; FRACTION ; MODELS |
| 资助项目 | National Key R & D Program of China[2022YFA1303802] ; Shanghai Municipal Science and Technology Major Project, National Natural Science Foundation of China[32271332] ; Shanghai Municipal Science and Technology Major Project, National Natural Science Foundation of China[31870801] ; Shanghai Municipal Science and Technology Major Project, National Natural Science Foundation of China[82404940] ; Fund of State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences[SIMM0120232001] ; High-level New R D Institute[2019B090904008] ; High-level Innovative Research Institute from Department of Science and Technology of Guangdong Province[2021B0909050003] ; Department of Science and Technology of Guangdong Province ; Zhongshan Municipal Bureau of Science and Technology |
| WOS研究方向 | Chemistry ; Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:001342897700001 |
| 出版者 | ELSEVIER SCI LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/314297]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | He, Fei; Ding, Kan |
| 作者单位 | 1.Lingang Lab, Shanghai, Peoples R China 2.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, SSIP Healthcare & Med Demonstrat Zone, Zhongshan 528400, Guangdong, Peoples R China 3.Huazhong Univ Sci & Technol, Tongji Med Coll Pharm, Hubei Key Lab Nat Med Chem & Resource Evaluat, Wuhan 430030, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, Carbohydrate Drug Res Ctr,Stake Key Lab Drug Res, CAS Key Lab Receptor Res,Glycochem & Glycobiol Lab, Shanghai 201203, Peoples R China 5.Nanjing Univ Chinese Med, Sch Chinese Mat Med, 138 Xianlin Ave, Nanjing 210029, Jiangsu, Peoples R China 6.ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai 201210, Peoples R China 7.Univ Chinese Acad Sci, Sch Pharm, 19A Yuquan Rd, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Meilin,Zong, Jianing,He, Fei,et al. Structural elucidation of an active arabinoglucan from Gomphrena globosa and its protection effect and mechanism against metabolic dysfunction-associated steatohepatitis[J]. CARBOHYDRATE POLYMERS,2025,348:15. |
| APA | Chen, Meilin.,Zong, Jianing.,He, Fei.,Zhou, Wanqi.,Liu, Renjie.,...&Ding, Kan.(2025).Structural elucidation of an active arabinoglucan from Gomphrena globosa and its protection effect and mechanism against metabolic dysfunction-associated steatohepatitis.CARBOHYDRATE POLYMERS,348,15. |
| MLA | Chen, Meilin,et al."Structural elucidation of an active arabinoglucan from Gomphrena globosa and its protection effect and mechanism against metabolic dysfunction-associated steatohepatitis".CARBOHYDRATE POLYMERS 348(2025):15. |
入库方式： OAI收割
来源：上海药物研究所
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