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A novel pectin-like polysaccharide from Crocus sativus targets Galectin-3 to inhibit hepatic stellate cells activation and liver fibrosis

文献类型：期刊论文

作者Tang, Bixi7,8; Jin, Can2,9; Li, Maoting2,4; Liu, Siqi5; Zhang, Xuemei8; Li, Jia1,2,3,5,7; Ding, Kan2,9; Zang, Yi6,7
刊名CARBOHYDRATE POLYMERS
出版日期2025-01-15
卷号348页码:16
关键词Liver fibrosis Galectin 3 Pectin Polysaccharide structure Hepatic stellate cell
ISSN号0144-8617
DOI10.1016/j.carbpol.2024.122826
通讯作者Zhang, Xuemei(xuemzhang@fudan.edu.cn) ; Li, Jia(jli@simm.ac.cn) ; Ding, Kan(dingkan@simm.ac.cn) ; Zang, Yi(yzang@lglab.ac.cn)
英文摘要Liver fibrosis may lead to cirrhosis and even cancer without effective clinical medicine available. Previous studies demonstrated that galactan-containing pectins or pectin-like polysaccharides might target Galectin-3 (Gal-3) to impede fibrosis. This research aims to discover novel pectin-like galactan to interfere with fibrosis for potential new drug development. Thus, we purify a novel homogeneous Rhamnogalacturonan-I like polysaccharide with galactan input, XHH2, from the Crocus sativus flower. XHH2 (MW: 35.7 kDa) consists of rhamnose, galacturonic acid, galactose, and arabinose in ratios of 6.6: 6.1: 25.2: 12.1. The backbone of XHH2 comprises 1, 3, 6-Gal and 1, 3, 4-GalA, with branches at O-3 of 1, 3, 6-Gal and O-4 of 1, 3, 4-GalA. O-3 branches include 1, 3-Gal, 1, 4-Gal, 1, 6-Gal, T-Gal, and T-Glc, while O-4 branches consist of 1, 2, 4-Rha, 1, 4-GalA, 1, 5-Ara, T-Ara, T-Gal, and Ta-HexA. Surface plasmon resonance measurement shows that XHH2 binds to both Gal-3 and integrin (31 to block Gal-3/integrin (31 interaction. Mechanism studies further suggest that XHH2 inactivates hepatic stellate cells (HSCs) via disturbing the Gal-3/Integrin-(31/FAK pathway to alleviate liver injury and fibrosis in vitro & in vivo. XHH2 shows a favorable drug safety in the acute toxicity test of oral administration of XHH2 in mice. Overall, XHH2 is an active ingredient against liver fibrosis by targeting the interaction between Gal-3 and Integrin-(31.
WOS关键词RHAMNOGALACTURONAN-I ; CONTRACTION ; VITRO ; VIVO
资助项目National Key Research and Development Program of China[2022YFA1303802] ; Shanghai Municipal Science and Technology Major Project[TM202301H002] ; National Natural Science Foundation of China[32271332] ; National Natural Science Foundation of China[31870801] ; National Natural Science Foundation of China[82130099] ; Shanghai Institute of Materia Medica, Chinese Academy of Sciences[CASIMM0120225006-2]
WOS研究方向Chemistry ; Polymer Science
语种英语
WOS记录号WOS:001347264100001
出版者ELSEVIER SCI LTD
源URL[http://119.78.100.183/handle/2S10ELR8/314300]  
专题中国科学院上海药物研究所
通讯作者Zhang, Xuemei; Li, Jia; Ding, Kan; Zang, Yi
作者单位1.Bohai Rim Adv Res Inst Drug Discovery, Shandong Lab Yantai Drug Discovery, Yantai 264117, Shandong, Peoples R China
2.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, Zhongshan 528437, Peoples R China
3.UCAS, Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Guangzhou, Peoples R China
4.Guizhou Med Univ, Sch Pharmaceut Sci, Guiyang 550025, Peoples R China
5.Nanjing Univ Chinese Med, Sch Chinese Mat Med, Nanjing 210023, Peoples R China
6.Lingang Lab, Shanghai 201203, Peoples R China
7.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Chem Biol, Shanghai 201203, Peoples R China
8.Fudan Univ, Sch Pharm, Dept Pharmacol, Shanghai 201203, Peoples R China
9.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Tang, Bixi,Jin, Can,Li, Maoting,et al. A novel pectin-like polysaccharide from Crocus sativus targets Galectin-3 to inhibit hepatic stellate cells activation and liver fibrosis[J]. CARBOHYDRATE POLYMERS,2025,348:16.
APA Tang, Bixi.,Jin, Can.,Li, Maoting.,Liu, Siqi.,Zhang, Xuemei.,...&Zang, Yi.(2025).A novel pectin-like polysaccharide from Crocus sativus targets Galectin-3 to inhibit hepatic stellate cells activation and liver fibrosis.CARBOHYDRATE POLYMERS,348,16.
MLA Tang, Bixi,et al."A novel pectin-like polysaccharide from Crocus sativus targets Galectin-3 to inhibit hepatic stellate cells activation and liver fibrosis".CARBOHYDRATE POLYMERS 348(2025):16.

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来源：上海药物研究所

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