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Integration of ion mobility-quadrupole time-of-flight mass spectrometry and triple quadrupole-ion trap mass spectrometry coupled with ultra-high performance liquid chromatography for characterizing and quantitatively assay the saponins of Panax quinquefolius flower

文献类型：期刊论文

作者Wang, Yu1,2; Hong, Li-li1,2; Sun, Meng-xiao1,2; Li, Xue1,2; Zou, Ya-dan1,2; Ding, Meng-xiang1,2; Zhang, Min1,2; Wang, Hong-da1,2; Guo, De-an1,2,3; Yang, Wen-zhi1,2
刊名MICROCHEMICAL JOURNAL
出版日期2024-12-01
卷号207页码:14
关键词Panax quinquefolius flower UHPLC/IM-QTOF-MS UHPLC/QTrap-MS Multi-component characterization Quantitative assay
ISSN号0026-265X
DOI10.1016/j.microc.2024.112124
通讯作者Wang, Hong-da(hdwang_1996@163.com) ; Guo, De-an(daguo@simm.ac.cn) ; Yang, Wen-zhi(wzyang0504@tjutcm.edu.cn)
英文摘要Panax quinquefolius (American ginseng) is globally popular serving as herbal medicine or tonifying agent. Compared with the root, studies on the flower of Panax quinquefolius (PQF) in respect of the multicomponent characterization and quality evaluation is scarce. This work was designed, by feat of two liquid chromatography-mass spectrometry platforms, to characterize and quantitatively assay the saponins of PQF. Ultra-high performance liquid chromatography/ion mobility-quadrupole time-of-flight mass spectrometry (UHPLC/IM-QTOF-MS) combined with data-independent high-definition MSE (HDMSE) was developed to acquire the structure information of PQF saponins. Consequently, efficient and more accurate identification (higher identification accuracy and much lower false positives rate) of the involved saponins were accomplished by the intelligent peak annotation workflows of the UNIFI bioinformatics platform incorporated with a "Multi-dimensional Information Library of Ginsenosides" (GinMIL). Totally 128 ginsenosides were identified or tentatively characterized, and 45 thereof were identified by comparing with the reference standards. Additionally, an efficient UHPLC/QTrap-MS (triple quadrupole-ion trap mass spectrometry) method enabling quantitative assay of 21 ginsenosides in PQF was established and validated by scheduled multiple reaction monitoring (sMRM). It gave low limit of detection (0.011-0.65 pg) and limit of quantification (0.053-4.1 pg), good linearity (R-2 > 0.99), intra-/inter-day precision (RSD < 13.6%), stability (RSD < 3.5%), repeatability (RSD < 7.5%), and recovery (RSD < 13.5%). It application to quantify 21 ginsenosides in 14 batches of PQF samples revealed differences in their saponin content. Conclusively, systematic multi-component characterization and multi-indicator quantitation of PQF were achieved, thus providing the methodological reference for the quality investigation of functional herbs.
WOS关键词TRADITIONAL CHINESE MEDICINE ; GINSENG ; GINSENOSIDES
资助项目Major Science & Technology Project of Tianjin[24ZXZKSY00010] ; Science & Technology Program of Haihe Laboratory of Modern Chinese Medicine[22HHZYSS00012] ; Postdoctoral Fellowship Program of CPSF[GZB20240531] ; China Postdoctoral Science Foundation-Tianjin Joint Support Program[2023T030TJ] ; Foundation of State Key Laboratory of Component-based Chinese Medicine[CBCM2023202] ; National Natural Science Foundation of China[82374030] ; Tianjin Outstanding Youth Fund[23JCJQJC00030]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:001358141500001
出版者ELSEVIER
源URL[http://119.78.100.183/handle/2S10ELR8/314527]  
专题中国科学院上海药物研究所
通讯作者Wang, Hong-da; Guo, De-an; Yang, Wen-zhi
作者单位1.Tianjin Univ Tradit Chinese Med, State Key Lab Chinese Med Modernizat, 10 Poyanghu Rd, Tianjin 301617, Peoples R China
2.Haihe Lab Modern Chinese Med, 10 Poyanghu Rd, Tianjin 301617, Peoples R China
3.Chinese Acad Sci, Natl Engn Lab TCM Standardizat Technol, Shanghai Inst Mat Med, 501 Haike Rd, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Yu,Hong, Li-li,Sun, Meng-xiao,et al. Integration of ion mobility-quadrupole time-of-flight mass spectrometry and triple quadrupole-ion trap mass spectrometry coupled with ultra-high performance liquid chromatography for characterizing and quantitatively assay the saponins of Panax quinquefolius flower[J]. MICROCHEMICAL JOURNAL,2024,207:14.
APA Wang, Yu.,Hong, Li-li.,Sun, Meng-xiao.,Li, Xue.,Zou, Ya-dan.,...&Yang, Wen-zhi.(2024).Integration of ion mobility-quadrupole time-of-flight mass spectrometry and triple quadrupole-ion trap mass spectrometry coupled with ultra-high performance liquid chromatography for characterizing and quantitatively assay the saponins of Panax quinquefolius flower.MICROCHEMICAL JOURNAL,207,14.
MLA Wang, Yu,et al."Integration of ion mobility-quadrupole time-of-flight mass spectrometry and triple quadrupole-ion trap mass spectrometry coupled with ultra-high performance liquid chromatography for characterizing and quantitatively assay the saponins of Panax quinquefolius flower".MICROCHEMICAL JOURNAL 207(2024):14.

入库方式： OAI收割

来源：上海药物研究所

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