Analysis of Herbal Constituents and In Vivo Pharmacokinetics of Gegen-Huangqi Decoction in Rat Plasma Using HPLC-Q-TOF-MS/MS and HPLC-QQQ-MS/MS
文献类型：期刊论文
| 作者 | Xu, Zhou2; Wang, Linwei1; Liu, Huan2; Tian, Xiaoting2; Wang, Yangyang2; Xu, Haibo2; Chen, Shuoji2; Chen, Mingcang2; Hu, Pei3; Huang, Chenggang2
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| 刊名 | BIOMEDICAL CHROMATOGRAPHY
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| 出版日期 | 2024-11-22 |
| 页码 | 16 |
| 关键词 | |
| ISSN号 | 0269-3879 |
| DOI | 10.1002/bmc.6046 |
| 通讯作者 | Hu, Pei(hupei@crjz.com) ; Huang, Chenggang(cghsimm@126.com) |
| 英文摘要 | The traditional Chinese medicine (TCM) formula, gegen-huangqi (GH) decoction, has been employed for over 200 years, notably for its therapeutic effects in treating conditions such as atherosclerosis and diabetes mellitus. Despite its long-standing use, comprehensive studies on the chemical constituents of GH and their in vivo pharmacokinetics (PK) remain limited. This study aimed to profile the bioactive compounds present in GH decoction and to explore their PK characteristics using HPLC-Q-TOF-MS/MS. Furthermore, a robust and validated analytical method was developed and applied to assess the PK of 11 plasma compounds using HPLC-QQQ-MS/MS. In total, 79 components were identified within the GH decoction. Pharmacokinetic analysis revealed distinct absorption and elimination profiles for compounds such as puerarin, daidzin, genistin, calycosin-7-O-beta-D-glucoside, and ononin, which exhibited profiles of quick absorption and excretion. Conversely, compounds such as daidzein, formononetin, genistein, astragaloside II, astragaloside IV, and calycosin showed more complex in vivo metabolic patterns, leading to multipeak concentration-time curves. All compounds, except astragalosides II and IV, were found to undergo significant hepatic clearance. These findings provide valuable insights into the pharmacokinetic behavior of GH decoction, which lays the foundation for further quality control, pharmacological exploration, and potential clinical application of this traditional remedy. |
| WOS关键词 | METABOLISM ; DAIDZIN |
| 资助项目 | National Science and Technology Major Project of China ; [2018ZX09731-016-003] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:001360630700001 |
| 出版者 | WILEY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/314577]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Hu, Pei; Huang, Chenggang |
| 作者单位 | 1.Shenyang Pharmaceut Univ, Sch Life Sci & Biopharmaceut, Shenyang, Liaoning, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai, Peoples R China 3.State Key Lab Modernizat Class & Famous Prescript, Nanchang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Zhou,Wang, Linwei,Liu, Huan,et al. Analysis of Herbal Constituents and In Vivo Pharmacokinetics of Gegen-Huangqi Decoction in Rat Plasma Using HPLC-Q-TOF-MS/MS and HPLC-QQQ-MS/MS[J]. BIOMEDICAL CHROMATOGRAPHY,2024:16. |
| APA | Xu, Zhou.,Wang, Linwei.,Liu, Huan.,Tian, Xiaoting.,Wang, Yangyang.,...&Huang, Chenggang.(2024).Analysis of Herbal Constituents and In Vivo Pharmacokinetics of Gegen-Huangqi Decoction in Rat Plasma Using HPLC-Q-TOF-MS/MS and HPLC-QQQ-MS/MS.BIOMEDICAL CHROMATOGRAPHY,16. |
| MLA | Xu, Zhou,et al."Analysis of Herbal Constituents and In Vivo Pharmacokinetics of Gegen-Huangqi Decoction in Rat Plasma Using HPLC-Q-TOF-MS/MS and HPLC-QQQ-MS/MS".BIOMEDICAL CHROMATOGRAPHY (2024):16. |
入库方式： OAI收割
来源：上海药物研究所
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