Raddeanin A suppresses intracellular 5Methylcytosine DNA modification engaged the metastasis of hepatocellular carcinoma
文献类型：期刊论文
| 作者 | Liu, Xin3,4,5,6; Xie, Xiaoyan1; Wang, Kangyu3,4,5; Liu, Xiaokang2; Gong, Jiyu3; Yang, Zizhao4,5; Li, Jiannan3 |
| 刊名 | JOURNAL OF ETHNOPHARMACOLOGY
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| 出版日期 | 2025-02-10 |
| 卷号 | 338页码:11 |
| 关键词 | Raddeanin a (RA) Hepatocellular carcinoma (HCC) DNA methyltransferase (DNMT) Tumorigenesis and metastasis Pharmacodynamic effect |
| ISSN号 | 0378-8741 |
| DOI | 10.1016/j.jep.2024.119036 |
| 通讯作者 | Yang, Zizhao(zzyang@shutcm.edu.cn) ; Li, Jiannan(lijiannan2007@126.com) |
| 英文摘要 | Ethnopharmacological relevance: The Anemonoides Raddeana (Rege) Holubhe is commonly employed in clinical practice as a traditional Chinese medicine for the treatment of conditions such as rheumatism and limb numbness. Raddeanin A (RA), an active compound derived from this Traditional Chinese Medicine (TCM), demonstrates specific anticancer properties against many tumorigeneses. However, the molecular mechanism underlying its effects on hepatocellular carcinoma (HCC) remains unexplored. Aim of the study: The aim of this study is to investigate the inhibitory effects of RA in human HCC stimulated cells and its impact on DNA methylation in tumor cells, as well as to elucidate the molecular mechanisms underlying RA's anti-tumor activity. Materials and methods: The inhibitory effects of RA on QGY-7703 and HepG2 cells were evaluated. The IC50 values were determined by employing non-linear sigmoidal curve fitting to analyze the normalized response. The impact of RA was investigated in cells overexpressing DNMT3A and DNMT3B. The effects of RA on cell cycle progression and apoptosis were assessed. Furthermore, the influence of RA on cellular methylation was determined, along with its effects on the expression levels of DNMT3A, DNMT3B, Bcl-2, Bax, and Caspase-3. Results: The findings demonstrate that RA induces cell cycle arrest at the G0/G1 phase and promotes apoptosis in hepatocellular carcinoma cells. Furthermore, RA effectively inhibits the invasion and migration of human HCC stimulated cells. The expression of DNMT3A and DNMT3B is downregulated by RA, effectively suppressing the intracellular 5mC DNA modification level. Moreover, the overexpression of these enzymes in RA-treated human HCC stimulated cells significantly impacts the overall 5mC level and hinders tumor metastasis by restricting migration and invasion. Conclusion: The RA compound acts as an antagonist against HCC by reducing intracellular DNA 5mC levels through mechanisms mediated by methyltransferase. Moreover, RA demonstrates the capacity to induce apoptosis in tumor cells, thereby exerting its anti-tumor effects. The findings of this study provide valuable insights for enhancing the pharmacodynamic efficacy of RA in HCC treatment. |
| WOS关键词 | APOPTOSIS ; METHYLATION ; DEMETHYLATION ; DNMT3A ; CELLS |
| 资助项目 | National Natural Science Foundation of China[U20A20405] ; National Natural Science Foundation of China[82104494] ; Shanghai Pujiang Program, China[23PJ1412300] ; Jilin Province Project of Science and Technology Development[20210204078YY] ; National Chinese Medicine Standardization Project[ZYBZH-Y-JL-25] |
| WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:001370510900001 |
| 出版者 | ELSEVIER IRELAND LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/314977]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Yang, Zizhao; Li, Jiannan |
| 作者单位 | 1.Changchun Univ Chinese Med, Dept Pharm, 3rd Affiliated Hosp, Changchun 130117, Peoples R China 2.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, Zhongshan 528400, Peoples R China 3.Changchun Univ Chinese Med, Sch Pharmaceut Sci, Key Lab Chinese Med Anal, Changchun 130117, Peoples R China 4.Shanghai Univ Tradit Chinese Med, Sch Pharm, Shanghai 201203, Peoples R China 5.Shanghai Univ Tradit Chinese Med, Dept Gen Surg, Peoples Hosp 7, Shanghai 200137, Peoples R China 6.Jilin Normal Univ, Postgrad Sch, Siping 136000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Xin,Xie, Xiaoyan,Wang, Kangyu,et al. Raddeanin A suppresses intracellular 5Methylcytosine DNA modification engaged the metastasis of hepatocellular carcinoma[J]. JOURNAL OF ETHNOPHARMACOLOGY,2025,338:11. |
| APA | Liu, Xin.,Xie, Xiaoyan.,Wang, Kangyu.,Liu, Xiaokang.,Gong, Jiyu.,...&Li, Jiannan.(2025).Raddeanin A suppresses intracellular 5Methylcytosine DNA modification engaged the metastasis of hepatocellular carcinoma.JOURNAL OF ETHNOPHARMACOLOGY,338,11. |
| MLA | Liu, Xin,et al."Raddeanin A suppresses intracellular 5Methylcytosine DNA modification engaged the metastasis of hepatocellular carcinoma".JOURNAL OF ETHNOPHARMACOLOGY 338(2025):11. |
入库方式： OAI收割
来源：上海药物研究所
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