Fluorogenic Labeling Probe for the Imaging of Endogenous β-Galactosidase Activity in Cancer and Senescent Cells
文献类型：期刊论文
| 作者 | Wang, Jing-Bo2,3; Zhao, Xue-Ru2,3,4; Hu, Xi-Le2,3; Zang, Yi5; Li, Jia4 ; He, Xiao-Peng1,2,3
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| 刊名 | ACS APPLIED MATERIALS & INTERFACES
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| 出版日期 | 2024-12-09 |
| 卷号 | 16期号:50页码:68918-68927 |
| 关键词 | fluorogenic probe labeling glycosidases cell imaging cancer senescence |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/acsami.4c15268 |
| 通讯作者 | Li, Jia(jli@simm.ac.cn) ; He, Xiao-Peng(xphe@ecust.edu.cn) |
| 英文摘要 | The sensitive detection of glycosidases in live cells is crucial to understanding their functional roles in disease progression. Here, we develop a fluorogenic labeling probe for beta-galactosidase (beta-Gal) based on a bright green-emitting fluorescent dye, fluorescein. Galactose was introduced to a fluoromethyl-substituted fluorescein derivative through a benzyl spacer, resulting in a quenched fluorescence due to spirocyclization of the dye. After removal of the galactosyl residue by beta-Gal, an similar to 210-fold enhanced green fluorescence (emission maximum at 524 nm) was detected, and the presence of other glycosidases and hydrolases did not produce false-positive signals. The probe was successfully used for imaging of the endogenous beta-Gal activity in cancer and senescent cells, and the imaging results agree with the beta-Gal expression level of the cells, as determined by Western blotting and polymerase chain reaction. Importantly, we demonstrated that upon hydrolysis of galactose, the fluoromethyl-substituted fluorescein derivative is covalently attached to adjacent proteins, both in solution and in live cells. This study offers a small-molecule probe for the sensitive monitoring of endogenous glycosidase activity. |
| WOS关键词 | IN-VIVO VISUALIZATION ; FLUORESCENT-PROBE ; GLUCURONIDASE ; STRATEGY ; TUMOR |
| 资助项目 | National Natural Science Foundation of China[92253306] ; National Natural Science Foundation of China[82130099] ; National Natural Science Foundation of China[22108077] ; Natural National Science Foundation of China (NSFC)[23490711600] ; International Cooperation Program of Shanghai Science and Technology[222201717003] ; Fundamental Research Funds for the Central Universities[B16017] ; Programme of Introducing Talents of Discipline to Universities[SKLDR-2023-KF-10] ; Open Funding Project of the State Key Laboratory of Bioreactor Engineering, State Key Laboratory of Drug Research ; State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University[2023-MEKLLC-MS/ZD-00*] ; Ministry of Education Key Laboratory on Signaling Regulation and Targeting Therapy of Liver Cancer (Naval Medical University) ; Research Center of Analysis and Test of East China University of Science and Technology |
| WOS研究方向 | Science & Technology - Other Topics ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:001372905300001 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/315007]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Li, Jia; He, Xiao-Peng |
| 作者单位 | 1.Eastern Hepatobiliary Surg Hosp, Natl Ctr Liver Canc, Int Cooperat Lab Signal Transduct, Shanghai 200438, Peoples R China 2.East China Univ Sci & Technol, Key Lab Adv Mat, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China 3.East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Sch Chem & Mol Engn, Joint Int Res Lab Precis Chem & Mol Engn, Shanghai 200237, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Chem Biol, Shanghai 201203, Peoples R China 5.Lingang Lab, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Jing-Bo,Zhao, Xue-Ru,Hu, Xi-Le,et al. Fluorogenic Labeling Probe for the Imaging of Endogenous β-Galactosidase Activity in Cancer and Senescent Cells[J]. ACS APPLIED MATERIALS & INTERFACES,2024,16(50):68918-68927. |
| APA | Wang, Jing-Bo,Zhao, Xue-Ru,Hu, Xi-Le,Zang, Yi,Li, Jia,&He, Xiao-Peng.(2024).Fluorogenic Labeling Probe for the Imaging of Endogenous β-Galactosidase Activity in Cancer and Senescent Cells.ACS APPLIED MATERIALS & INTERFACES,16(50),68918-68927. |
| MLA | Wang, Jing-Bo,et al."Fluorogenic Labeling Probe for the Imaging of Endogenous β-Galactosidase Activity in Cancer and Senescent Cells".ACS APPLIED MATERIALS & INTERFACES 16.50(2024):68918-68927. |
入库方式： OAI收割
来源：上海药物研究所
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