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The structure elucidation and alleviating effect on liver fibrosis in vivo of pectin-like polysaccharide isolated from Buddleja officinalis

文献类型：期刊论文

作者Zong, Jianing3,5; Li, Yun4,5; Zhou, Wanqi5,6,7; Mao, Mengfei4,5; Xu, Xin4,5; Cai, Simin1,5; Li, Meixia4,5; Ding, Kan2,3,4,5,6,7
刊名INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
出版日期2025
卷号284页码:14
关键词Buddleja officinalis Polysaccharides Pectin Liver fibrosis
ISSN号0141-8130
DOI10.1016/j.ijbiomac.2024.137936
通讯作者Li, Meixia(limei@simm.ac.cn) ; Ding, Kan(dingkan@simm.ac.cn)
英文摘要Buddleja officinalis has been used as a traditional Chinese medicine for years. Although evidence has demonstrated it can enhance liver function, the active material basis remains unknown. We hypothesize polysaccharides from Buddleja officinalis may be the active material against liver disease. Herein, we elucidated the structure of a novel pectin-like polysaccharide designed BOM0.05S2 with a molecular weight of 13.6 kDa. Combined with endo-1, 4-(3-Mannanase degradation, we found its backbone consists of alternate 1, 2, 4-linked alpha-Rhap and 1, 4-linked alpha-GalpA (RG-I type pectin) and mannoglucan, with branches of 1, 4-, 1, 6and 1, 3, 6linked (3-Galp, T-, 1, 5- and 1, 3, 5-linked alpha-Araf, T-linked (3-Manp and T-linked alpha-Glcp substituted at C-4 of 1, 2, 4-linked alpha-Rhap and C-6 of 1, 4, 6-linked alpha-Glcp. As speculated, BOM0.05S2 showed a significant improvement on carbon tetrachloride (CCl4)-induced liver damage in mice. Bioactivity test showed that BOM0.05S2 reduced AST, ALT and four indexes of liver fibrosis including LN, HA, IV-C, PC-III. Further, we demonstrated that BOM0.05S2 attenuated the collagenous fiber and alpha-SMA in liver. These findings highlight the potential of BOM0.05S2 as a lead compound for the treatment of liver fibrosis.
WOS关键词NONALCOHOLIC STEATOHEPATITIS ; INHIBITION ; EXTRACT ; FLOWERS ; PHASE-3 ; CELLS ; EYE
资助项目National Key Research and Devel-opment Program of China[2022YFA1303802] ; National Natural Science Foundation of China[82341097] ; National Natural Science Foundation of China[32271332] ; National Natural Science Foundation of China[32471328] ; High-level Inno-vative Research Institute[2021B0909050003] ; Department of Science and Technology of Guangdong Province
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种英语
WOS记录号WOS:001371443500001
出版者ELSEVIER
源URL[http://119.78.100.183/handle/2S10ELR8/315016]  
专题中国科学院上海药物研究所
通讯作者Li, Meixia; Ding, Kan
作者单位1.Henan Univ, Sch Pharm, Kaifeng 475004, Henan, Peoples R China
2.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, Zhongshan 528400, Peoples R China
3.Nanjing Univ Chinese Med, Sch Chinese Mat Med, 138 Xianlin Ave, Nanjing 210029, Jiangsu Provinc, Peoples R China
4.Univ Chinese Acad Sci, Sch Pharm, 19A Yuquan Rd, Beijing 100049, Peoples R China
5.Chinese Acad Sci, Shanghai Inst Mat Med, Carbohydrate Drug Res Ctr, Glycochem & Glycobiol Lab, Shanghai 201203, Peoples R China
6.ShanghaiTech Univ, Sch Phys Sci & Technol, Shanghai 201210, Peoples R China
7.Lingang Lab, Shanghai, Peoples R China
推荐引用方式
GB/T 7714		 Zong, Jianing,Li, Yun,Zhou, Wanqi,et al. The structure elucidation and alleviating effect on liver fibrosis in vivo of pectin-like polysaccharide isolated from Buddleja officinalis[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2025,284:14.
APA Zong, Jianing.,Li, Yun.,Zhou, Wanqi.,Mao, Mengfei.,Xu, Xin.,...&Ding, Kan.(2025).The structure elucidation and alleviating effect on liver fibrosis in vivo of pectin-like polysaccharide isolated from Buddleja officinalis.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,284,14.
MLA Zong, Jianing,et al."The structure elucidation and alleviating effect on liver fibrosis in vivo of pectin-like polysaccharide isolated from Buddleja officinalis".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 284(2025):14.

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来源：上海药物研究所

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