A Mitochondria-Targeting and Peroxynitrite-Activatable Ratiometric Fluorescent Probe for Precise Tracking of Oxidative Stress-Induced Mitophagy
文献类型：期刊论文
| 作者 | Chai, Xianzhi2,3; Ma, Xiuhua2; Sun, Lu-Lu1; Hu, Yuqing2; Zhang, Weijian2; Zhang, Shiyao2; Zhou, Jiaqi2; Zhu, Liangliang3; Han, Hai-Hao1,4,5; He, Xiao-Peng6,7,8 |
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2024-12-09 |
| 卷号 | 96期号:51页码:20161-20168 |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.4c03759 |
| 通讯作者 | Han, Hai-Hao(hanhaihao@simm.ac.cn) ; He, Xiao-Peng(xphe@ecust.edu.cn) |
| 英文摘要 | Mitochondria are the energy factory of cells and can be easily damaged by reactive oxygen species (ROS) because of the frequent occurrence of oxidative stress. Abnormality in mitophagy is often associated with many diseases including inflammation, cancer, and aging. While previously developed fluorescent probes mainly focus on detecting just ROS or mitophagy, quite rare studies have endeavored to comprehensively capture the entire mitophagic process, encompassing both the production of ROS and the induction of mitophagy. Herein, we report a new ratiometric fluorescent probe NA-DP for tracking peroxynitrite (ONOO-) as well as the subsequent oxidative stress-induced mitophagy. To a naphthalimide-based dye, an ONOO--responsive diphenyl phosphinate moiety and the mitochondria-targeting triphenylphosphonium group were attached. The probe showed a highly selective response to ONOO- through an addition-elimination reaction with diphenyl phosphinate. Owing to its outstanding pH stability and organelle-targeting ability, NA-DP was successfully used to detect mitophagy induced by oxidative stress after the generation of ONOO-. In the meantime, the probe was also used to track starvation-induced mitophagy and indicate that starvation-induced mitophagy is independent of ONOO-. Therefore, NA-DP has the ability to precisely track oxidative stress-induced mitophagy by distinguishing it from starvation-induced mitophagy. This study offers a new chemical tool to study the relationship between ROS generation and mitophagy. |
| WOS关键词 | AUTOPHAGY |
| 资助项目 | Key Technology Research and Development Program of Shandong Province[22308134] ; Key Technology Research and Development Program of Shandong Province[22107029] ; Key Technology Research and Development Program of Shandong Province[22377135] ; Key Technology Research and Development Program of Shandong Province[92253306] ; Key Technology Research and Development Program of Shandong Province[82130099] ; National Natural Science Foundation of China[BK20220644] ; Natural Science Foundation of Jiangsu Province[tsqn202312305] ; Taishan Scholars Program ; Young Elite Scientists Sponsorship Program by Chinese Chemical Society[2023JCYJ059] ; Fundamental Research Projects of Science & Technology Innovation and development Plan in Yantai City[SYS202205] ; Shandong Laboratory Program[2024CXPT028] ; Key R&D Program of Shandong[2018SHZDZX03] ; Shanghai Municipal Science and Technology Major Project[23490711600] ; International Cooperation Program of Shanghai Science and Technology[222201717003] ; Fundamental Research Funds for the Central Universities[B16017] ; Program of Introducing Talents of Discipline to Universities[SKLDR-2023-KF-10] ; State Key Laboratory of Bioreactor Engineering, State Key Laboratory of Drug Research[2023-MEKLLC-MS/ZD-00*] ; Ministry of Education Key Laboratory on signaling Regulation and Targeting Therapy of Liver Cancer (Naval Medical University) |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001374186100001 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/315022]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Han, Hai-Hao; He, Xiao-Peng |
| 作者单位 | 1.Bohai Rim Adv Res Inst Drug Discovery, Shandong Lab Yantai Drug Discovery, Yantai 264117, Shandong, Peoples R China 2.Jiangsu Univ Sci & Technol, Sch Environm & Chem Engn, Zhenjiang 212100, Jiangsu, Peoples R China 3.Fudan Univ, Dept Macromol Sci, State Key Lab Mol Engn Polymers, Shanghai 200438, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, Mol Imaging Ctr, Stake Key Lab Chem Biol, Shanghai 201203, Peoples R China 5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 6.East China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China 7.East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Ctr Materiobiol & Dynam Chem, Sch Chem & Mol Engn,Joint Int Res Lab Precis Chem, Shanghai 200237, Peoples R China 8.Eastern Hepatobiliary Surg Hosp, Natl Ctr Liver Canc, Int Cooperat Lab Signal Transduct, Shanghai 200438, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chai, Xianzhi,Ma, Xiuhua,Sun, Lu-Lu,et al. A Mitochondria-Targeting and Peroxynitrite-Activatable Ratiometric Fluorescent Probe for Precise Tracking of Oxidative Stress-Induced Mitophagy[J]. ANALYTICAL CHEMISTRY,2024,96(51):20161-20168. |
| APA | Chai, Xianzhi.,Ma, Xiuhua.,Sun, Lu-Lu.,Hu, Yuqing.,Zhang, Weijian.,...&He, Xiao-Peng.(2024).A Mitochondria-Targeting and Peroxynitrite-Activatable Ratiometric Fluorescent Probe for Precise Tracking of Oxidative Stress-Induced Mitophagy.ANALYTICAL CHEMISTRY,96(51),20161-20168. |
| MLA | Chai, Xianzhi,et al."A Mitochondria-Targeting and Peroxynitrite-Activatable Ratiometric Fluorescent Probe for Precise Tracking of Oxidative Stress-Induced Mitophagy".ANALYTICAL CHEMISTRY 96.51(2024):20161-20168. |
入库方式： OAI收割
来源：上海药物研究所
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