Characterization of acidic lysine acylations in mycobacteria
文献类型：期刊论文
| 作者 | Ye, Tong1,4; Wang, Danfeng2,3; Sun, Yewen2; Xie, Shuyu1,4; Liu, Tianqi2; Tian, Nana2,3; Tan, Minjia1,2,3,4 ; Xu, Jun-Yu1,2,3,4
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| 刊名 | FRONTIERS IN MICROBIOLOGY
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| 出版日期 | 2024-12-10 |
| 卷号 | 15页码:12 |
| 关键词 | |
| DOI | 10.3389/fmicb.2024.1503184 |
| 通讯作者 | Tan, Minjia(mjtan@simm.ac.cn) ; Xu, Jun-Yu(jyxu@simm.ac.cn) |
| 英文摘要 | Introduction Protein acetylation is an extensively investigated post-translational modification (PTM). In addition to lysine acetylation, three new types of lysine acylations characterized by the presence of an acidic carboxylic group have been recently identified and validated. These included lysine malonylation (Kmal), lysine succinylation (Ksucc) and lysine glutarylation (Kglu). Pathogens belonging to the genus Mycobacterium elicit severe diseases in mammalian hosts through the modulation of energy metabolism pathways. Throughout this process, malonyl-CoA, succinyl-CoA and glutaryl-CoA are important intermediates in metabolic pathways, including the tricarboxylic acid (TCA) cycle, amino acid and lipid metabolism. These short-chain acyl-CoAs serve as substrates for corresponding acidic lysine acylation reactions. However, the landscape of these acyl-CoAs dependent acidic lysine acylomes remains unclear.Methods We used the high-affinity antibody enrichment combined with high-resolution LC-MS/MS analysis to systematically investigate the global proteomic characteristics of the three acidic lysine acylations in Mycobacterium smegmatis. Subsequently, we employed in vitro enzymatic assays to validate the functional impact of acylated substrates, adenylate kinase and proteasome-associated ATPase. Furthermore, we investigated the effects of overexpressing these two substrates on the in vitro growth of Mycobacterium smegmatis, its invasion of THP-1 cells, and the influence on inflammatory cytokines.Results We systematically investigated the global substrate characterization of 1,703 lysine malonylated sites, 5,320 lysine succinylated sites and 269 lysine glutarylated sites in the non-pathogenic model strain Mycobacterium smegmatis. Bioinformatics analysis demonstrated a correlation between these acidic lysine acylations and the functional roles of ribosomes, in addition to their roles in various metabolic pathways. Furthermore, we investigated the impact of lysine acylations on the functional activity of adenylate kinase and proteasome-associated ATPase, as well as their roles in mycobacterial infection process.Discussion Collectively, our study provided an important resource on substrate characterization and functional regulation of acidic lysine acylations in Mycobacterium smegmatis, giving valuable insights into their interrelation with the biology of infectious process. |
| WOS关键词 | ADENYLATE KINASE ; SUCCINYLATION ; IDENTIFICATION ; GLUTARYLATION ; PROKARYOTES |
| 资助项目 | National Key Research and Development Program of China[2023YFA1800400] ; National Key Research and Development Program of China[2023YFA1800403] ; Program of Shanghai Academic Research Leader[22XD1420900] ; Innovative Research Team of High-Level Local Universities in Shanghai[SHSMU-ZDCX20212700] ; National Natural Science Foundation of China[32322048] ; Young Elite Scientists Sponsorship Program by CAST[2022QNRC001] ; Shanghai Rising-Star Program[22QA1411100] ; Youth Innovation Promotion Association[CAS2021276] ; Sanofi scholarship program, Guangdong High-level New R&D Institute, China[:2019B090904008] ; Guangdong High-level Innovative Research Institute, China[:2021B0909050003] |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:001382271800001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/315160]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Tan, Minjia; Xu, Jun-Yu |
| 作者单位 | 1.Nanjing Univ, Chinese Med, Sch Chinese Mat Med, Nanjing, Jiangsu, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, Zhongshan Inst Drug Discovery, Zhongshan, Guangdong, Peoples R China 3.Zunyi Med Univ, Sch Pharm, Zunyi, Peoples R China 4.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ye, Tong,Wang, Danfeng,Sun, Yewen,et al. Characterization of acidic lysine acylations in mycobacteria[J]. FRONTIERS IN MICROBIOLOGY,2024,15:12. |
| APA | Ye, Tong.,Wang, Danfeng.,Sun, Yewen.,Xie, Shuyu.,Liu, Tianqi.,...&Xu, Jun-Yu.(2024).Characterization of acidic lysine acylations in mycobacteria.FRONTIERS IN MICROBIOLOGY,15,12. |
| MLA | Ye, Tong,et al."Characterization of acidic lysine acylations in mycobacteria".FRONTIERS IN MICROBIOLOGY 15(2024):12. |
入库方式： OAI收割
来源：上海药物研究所
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