Comprehensive Optimization of Packing Parameters for Hydraulic-Packed Capillary Columns
文献类型：期刊论文
| 作者 | Hu, Chunqiu1,5,6; Fu, Hang3,4; Wang, Lulu3; Gao, Yuan3; Li, Ruidong5; Li, Zhen2; Li, Shuai2; Xie, Xiaowang2; Ren, Chongxi2; Guo, Fang2 |
| 刊名 | RAPID COMMUNICATIONS IN MASS SPECTROMETRY
 |
| 出版日期 | 2025-04-15 |
| 卷号 | 39期号:7页码:11 |
| 关键词 | capillary columns hydraulic packing liquid chromatography-mass spectrometry proteomics packing efficiency |
| ISSN号 | 0951-4198 |
| DOI | 10.1002/rcm.9966 |
| 通讯作者 | Zhai, Linhui(zhailinhui@tongji.edu.cn) |
| 英文摘要 | RationaleThe performance of the capillary column directly impacts the separation efficiency of complex sample in liquid chromatography-mass spectrometry-based proteomics studies. The hydraulic packing system offers an effective solution by reducing packing time and expediting the preparation process of column preparation. However, its operational complexity and strict parameter regulation requirements hinder efficient application.MethodsThis study developed an innovative device for the hydraulic capillary column packing, followed by a systematic optimization and evaluation of crucial parameters such as packing pressure, magnetic stirring rate, concentration of solid-phase material, and choice of packing solvent in the capillary column packing process.ResultsThe comprehensive evaluation of the prepared capillary columns showed that the hydraulic packing method enabled rapid and large-scale preparation, while also demonstrating exceptional stability and reproducibility.ConclusionsThe proposed hydraulic capillary column packing strategy in this study holds theoretical and practical implications for advancing the technology of column preparation, enhancing separation efficiency, and expanding the depth of analysis in proteomics research. |
| WOS关键词 | PERFORMANCE ; SEPARATION ; EFFICIENCY ; INSIGHTS |
| 资助项目 | Program of Shanghai Academic Research Leader ; National Natural Science Foundation of China[32171434] ; [22XD1420900] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Spectroscopy |
| 语种 | 英语 |
| WOS记录号 | WOS:001386028600001 |
| 出版者 | WILEY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/315267]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Zhai, Linhui |
| 作者单位 | 1.Tongji Univ, Canc Ctr, Sch Med, Shanghai, Peoples R China 2.Shanghai Easymass Co Ltd, Shanghai, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai, Peoples R China 4.Nanjing Univ Chinese Med, New Sch Chinese Mat Med, Nanjing, Peoples R China 5.Jiangsu Ocean Univ, Coll Pharm, Jiangsu Key Lab Marine Pharmaceut Cpd Screening, Lianyungang, Peoples R China 6.Tongji Univ, Shanghai Peoples Hosp 4, Translat Res Inst Brain & Brain Like Intelligence, Sch Med, Shanghai, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Chunqiu,Fu, Hang,Wang, Lulu,et al. Comprehensive Optimization of Packing Parameters for Hydraulic-Packed Capillary Columns[J]. RAPID COMMUNICATIONS IN MASS SPECTROMETRY,2025,39(7):11. |
| APA | Hu, Chunqiu.,Fu, Hang.,Wang, Lulu.,Gao, Yuan.,Li, Ruidong.,...&Zhai, Linhui.(2025).Comprehensive Optimization of Packing Parameters for Hydraulic-Packed Capillary Columns.RAPID COMMUNICATIONS IN MASS SPECTROMETRY,39(7),11. |
| MLA | Hu, Chunqiu,et al."Comprehensive Optimization of Packing Parameters for Hydraulic-Packed Capillary Columns".RAPID COMMUNICATIONS IN MASS SPECTROMETRY 39.7(2025):11. |
入库方式： OAI收割
来源：上海药物研究所
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