Structure Elucidation and Immunostimulatory Activity Evaluation of a Galactoglucan from Alpinia officinarum Hance
文献类型：期刊论文
| 作者 | Xu, Zhou2; Xiong, Yanxia1,3; Hu, Pei3; Chen, Long3; Wan, Jianhua3; Huang, Chenggang2,3 ; Liu, Wenjun3
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| 刊名 | FOODS
 |
| 出版日期 | 2024-12-01 |
| 卷号 | 13期号:24页码:15 |
| 关键词 | |
| DOI | 10.3390/foods13244019 |
| 通讯作者 | Huang, Chenggang(cghsimm@126.com) ; Liu, Wenjun(crjzliuwenjun@163.com) |
| 英文摘要 | Alpinia officinarum Hance has a medicinal history of thousands of years in treating cough, diabetes, and gastrointestinal system diseases, and it is also a medicine food homology (MFH) plant in China. To evaluate the pharmacological activities of polysaccharides from the rhizomes of A. officinarum, polysaccharides were initially obtained by hot-water extraction and the ethanol precipitation method. A homogenous polysaccharide designated as AOP-w was isolated by a DE-52 column. The proposed structure was elucidated and the immunoregulatory effects on RAW 264.7 macrophage cells were evaluated. The results showed that AOP-w had a molecular weight of 5.26 kDa, and mainly consisted of galactose and glucose (molar ratio of 0.12:0.88). Its backbone comprised alpha-(1 -> 4)-Glcp, alpha-(1 -> 4,6)-Glcp and beta-(1 -> 3,4)-Galp residues, terminated by alpha-(1 -> 6)-Glcp and T-Glcp residues. AOP-w was nontoxic to RAW 264.7 cells, but demonstrated promotion in cell proliferation within a 100 mu g/mL concentration. The immunostimulatory effects of AOP-w were confirmed by the elevated NO production of AOP-w-treated cells. Moreover, the RNAseq was conducted and the results showed that AOP-w may activate the TNF and NF-kappa B signaling pathways by binding to Toll-like receptors, thereby affecting the immune modulatory activity of RAW264.7 cells. These results suggest a high potential of AOP-w from A. officinarum for immunotherapeutic applications. |
| WOS关键词 | POLYSACCHARIDES |
| 资助项目 | National Science and Technology Major Project of China ; [2019ZX09201001-001-008] |
| WOS研究方向 | Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:001384367500001 |
| 出版者 | MDPI |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/315285]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Huang, Chenggang; Liu, Wenjun |
| 作者单位 | 1.East China Univ Sci & Technol, Sch Chem Engn, 130 Mei Long Rd, Shanghai 200237, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, 501 Hai Ke Rd, Shanghai 201203, Peoples R China 3.State Key Lab Modernizat Class & Famous Prescript, 1899 Meiling Rd, Nanchang 330103, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Zhou,Xiong, Yanxia,Hu, Pei,et al. Structure Elucidation and Immunostimulatory Activity Evaluation of a Galactoglucan from Alpinia officinarum Hance[J]. FOODS,2024,13(24):15. |
| APA | Xu, Zhou.,Xiong, Yanxia.,Hu, Pei.,Chen, Long.,Wan, Jianhua.,...&Liu, Wenjun.(2024).Structure Elucidation and Immunostimulatory Activity Evaluation of a Galactoglucan from Alpinia officinarum Hance.FOODS,13(24),15. |
| MLA | Xu, Zhou,et al."Structure Elucidation and Immunostimulatory Activity Evaluation of a Galactoglucan from Alpinia officinarum Hance".FOODS 13.24(2024):15. |
入库方式： OAI收割
来源：上海药物研究所
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