A peptide-centric local stability assay enables proteome-scale identification of the protein targets and binding regions of diverse ligands
文献类型：期刊论文
| 作者 | Li, Kejia1,2,6; Chen, Shijie3; Wang, Keyun1; Wang, Yan1; Xue, Lianji1,2; Ye, Yuying1,2; Fang, Zheng1,2; Lyu, Jiawen1,2,7; Zhu, Haiyang1,2; Li, Yanan1 |
| 刊名 | NATURE METHODS
 |
| 出版日期 | 2024-12-10 |
| 页码 | 17 |
| ISSN号 | 1548-7091 |
| DOI | 10.1038/s41592-024-02553-7 |
| 英文摘要 | By using a limited-proteolysis strategy that employs a large amount of trypsin to generate peptides directly from native proteins, we found that ligand-induced protein local stability shifts can be sensitively detected on a proteome-wide scale. This enabled us to develop the peptide-centric local stability assay, a modification-free approach that achieves unprecedented sensitivity in proteome-wide target identification and binding-region determination. We demonstrate the broad applications of the peptide-centric local stability assay by investigating interactions across various biological contexts. |
| WOS关键词 | STRUCTURAL-CHANGES ; DRUG ; STABILIZATION |
| 资助项目 | National Natural Science Foundation of China (National Science Foundation of China)[2021YFA1302601] ; National Key Research and Development Program of China[92153302] ; National Key Research and Development Program of China[22437007] ; National Key Research and Development Program of China[22137002] ; National Key Research and Development Program of China[92253303] ; National Key Research and Development Program of China[22204033] ; National Natural Science Foundation of China[2023JJ11CG006] ; Dalian Science and Technology Innovation Fund ; DICP, CAS[I202109] ; DICP, CAS[DICP I202139] ; DICP[ZYYCXTD-202004] ; National Multidisciplinary Innovation Team of Traditional Chinese Medicine from National Administration of Traditional Chinese Medicine[2022279] ; Youth Innovation Promotion Association of CAS |
| WOS研究方向 | Biochemistry & Molecular Biology |
| WOS记录号 | WOS:001374459300001 |
| 出版者 | NATURE PORTFOLIO |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/315037]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Luo, Cheng; Ye, Mingliang |
| 作者单位 | 1.Chinese Acad Sci, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, State Key Lab Med Prote,Dalian Inst Chem Phys, Dalian, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Drug Discovery & Design Ctr, Ctr Chem Biol,State Key Lab Drug Res, Shanghai, Peoples R China 4.Dalian Univ Technol, Sch Bioengn, MOE Key Lab Biointelligent Mfg, Dalian, Peoples R China 5.Guizhou Med Univ, Affiliated Hosp, Sch Pharm, Dept Hepatobiliary Surg, Guiyang, Peoples R China 6.European Mol Biol Lab, Genome Biol Unit, Heidelberg, Germany 7.Karolinska Inst, Dept Oncol Pathol, Stockholm, Sweden |
| 推荐引用方式 GB/T 7714 | Li, Kejia,Chen, Shijie,Wang, Keyun,et al. A peptide-centric local stability assay enables proteome-scale identification of the protein targets and binding regions of diverse ligands[J]. NATURE METHODS,2024:17. |
| APA | Li, Kejia.,Chen, Shijie.,Wang, Keyun.,Wang, Yan.,Xue, Lianji.,...&Ye, Mingliang.(2024).A peptide-centric local stability assay enables proteome-scale identification of the protein targets and binding regions of diverse ligands.NATURE METHODS,17. |
| MLA | Li, Kejia,et al."A peptide-centric local stability assay enables proteome-scale identification of the protein targets and binding regions of diverse ligands".NATURE METHODS (2024):17. |
入库方式： OAI收割
来源：上海药物研究所
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