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Diazonium-based derivatization for enhanced detection of phosphorylated metabolites by LC-MS in cells

文献类型：期刊论文

作者Wang, Yikang2,3; Lin, Feifei1; Zhu, Guozheng2,3; Zhou, Xiaoxue2,3; Hu, Youhong1,2,3; Liu, Jia1,2,3
刊名JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
出版日期2025-03-15
卷号255页码:7
关键词Phosphorylated metabolite Derivatization Chromatographic separation Quantitative analysis LC-MS
ISSN号0731-7085
DOI10.1016/j.jpba.2024.116642
通讯作者Liu, Jia(jia.liu@simm.ac.cn)
英文摘要Phosphorylated small molecule metabolites play crucial roles in physiological processes such as glycogen metabolism and inflammation regulation. However, their high polarity, structural similarity, poor chromatographic separation, and weak mass spectrometric signals make their accurate quantification challenging, thereby hindering the study of related metabolic mechanisms and diseases. To address these challenges, we developed a novel derivatization reagent, DMQX (5-diazomethane quinoxaline), and combined it with liquid chromatography-mass spectrometry (LC-MS). This approach achieved baseline separation of five groups of isomers and enabled the quantification of 24 phosphorylated metabolites, providing comprehensive coverage of these metabolites in biological pathways. We applied this method to quantify 21 endogenous phosphorylated metabolites in HepG2 cells with and without vesicular stomatitis virus infection, demonstrating the potential of this analytical approach for advancing the study of metabolic mechanisms through quantitative analysis of phosphorylated metabolites in biological samples.
WOS关键词MASS-SPECTROMETRY ; QUANTITATIVE-ANALYSIS ; SEPARATION ; PHOSPHATE ; ACIDS
WOS研究方向Chemistry ; Pharmacology & Pharmacy
语种英语
WOS记录号WOS:001391301400001
出版者ELSEVIER
源URL[http://119.78.100.183/handle/2S10ELR8/315582]  
专题中国科学院上海药物研究所
通讯作者Liu, Jia
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
2.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China
3.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Hangzhou 310058, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Yikang,Lin, Feifei,Zhu, Guozheng,et al. Diazonium-based derivatization for enhanced detection of phosphorylated metabolites by LC-MS in cells[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2025,255:7.
APA Wang, Yikang,Lin, Feifei,Zhu, Guozheng,Zhou, Xiaoxue,Hu, Youhong,&Liu, Jia.(2025).Diazonium-based derivatization for enhanced detection of phosphorylated metabolites by LC-MS in cells.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,255,7.
MLA Wang, Yikang,et al."Diazonium-based derivatization for enhanced detection of phosphorylated metabolites by LC-MS in cells".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 255(2025):7.

入库方式： OAI收割

来源：上海药物研究所

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