Pharmacodynamic Mechanisms of Cicadae Periostracum in Parkinson's Disease: A Metabolomics-Based Study
文献类型：期刊论文
| 作者 | Li, Mengmeng1,2; Xiong, Fuyu1,2; Wu, Shifei1; Wei, Wenlong1; Wang, Hanze1,2; Qiao, Yajun1; Guo, Dean1,2
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| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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| 出版日期 | 2025 |
| 卷号 | 26期号:2页码:18 |
| 关键词 | Parkinson's disease Cicadae Periostracum metabolomics oxidative stress mechanisms of action |
| ISSN号 | 1661-6596 |
| DOI | 10.3390/ijms26020544 |
| 通讯作者 | Guo, Dean(daguo@simm.ac.cn) |
| 英文摘要 | Cicadae Periostracum (CP) is a traditional Chinese animal-derived medicine with the potential to treat Parkinson's disease (PD). This study aims to explore the pharmacodynamic mechanisms of CP against PD-based on metabolomics technology and provide a theoretical basis for developing new anti-PD medicine. First, MPP+-induced SH-SY5Y cells were used to evaluate the anti-PD activity of CP. In the animal study, an MPTP-induced PD mouse model was employed to assess CP's therapeutic effects. Immunofluorescence (IF) staining and Western blotting (WB) were used to evaluate its neuroprotective activity on neurons. A Serum metabolomics analysis was conducted to examine CP's regulatory effects on metabolites and to identify vital metabolic pathways. Finally, cellular experiments were performed to validate the critical pathways. Cellular activity experiments demonstrated that CP mitigates MPP+-induced SH-SY5Y cytotoxicity, inhibits apoptosis, and restores mitochondrial homeostasis. Animal experiments revealed that CP significantly alleviates dyskinesia in PD mice, enhances motor performance, and restores neuronal integrity while reducing alpha-synuclein (alpha-syn) aggregation in the striatum (STR), showing its strong anti-PD effect. Metabolomic analysis revealed that CP can significantly improve the metabolic disorders of ten biomarkers that are mainly involved in amino acid metabolism and fatty acid beta-oxidation and are closely related to oxidative stress pathways. Finally, pathway verification was performed, and the results show that CP exerted neuroprotective effects against PD through the dual signaling pathways of Bcl-2/Bax/Caspase-3 and Nrf2/HO-1. This study provides a comprehensive strategy for elucidating the mechanisms by which CP exerts its therapeutic effects against PD, highlighting its potential in developing anti-PD drugs. |
| WOS关键词 | N-ACETYLDOPAMINE DERIVATIVES ; MITOCHONDRIAL DYSFUNCTION ; OXIDATIVE STRESS |
| 资助项目 | Qi-Huang Chief Scientist Program of National Administration of Traditional Chinese Medicine (2020) ; Qi-Huang Chief Scientist Program of National Administration of Traditional Chinese Medicine[82130111] ; Key Program of the National Natural Science Foundation of China |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001404364600001 |
| 出版者 | MDPI |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/315947]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Guo, Dean |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Res Ctr TCM Standardizat Technol, Shanghai 201203, Peoples R China 2.Changchun Univ Chinese Med, Coll Pharmaceut Sci, Changchun 130117, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Mengmeng,Xiong, Fuyu,Wu, Shifei,et al. Pharmacodynamic Mechanisms of Cicadae Periostracum in Parkinson's Disease: A Metabolomics-Based Study[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2025,26(2):18. |
| APA | Li, Mengmeng.,Xiong, Fuyu.,Wu, Shifei.,Wei, Wenlong.,Wang, Hanze.,...&Guo, Dean.(2025).Pharmacodynamic Mechanisms of Cicadae Periostracum in Parkinson's Disease: A Metabolomics-Based Study.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,26(2),18. |
| MLA | Li, Mengmeng,et al."Pharmacodynamic Mechanisms of Cicadae Periostracum in Parkinson's Disease: A Metabolomics-Based Study".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26.2(2025):18. |
入库方式： OAI收割
来源：上海药物研究所
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