Luteolin alleviates olfactory dysfunction in eosinophilic chronic rhinosinusitis through modulation of the TLR4/NF-κB signaling pathway
文献类型：期刊论文
| 作者 | Shi, Jiali3,4; Dai, Li3; Gu, Jun2; Liu, Lu1; Chen, Yunhua2; Jiang, Yiming3; Yu, Yafeng4 |
| 刊名 | INTERNATIONAL IMMUNOPHARMACOLOGY
 |
| 出版日期 | 2025-02-20 |
| 卷号 | 148页码:15 |
| 关键词 | Luteolin ECRS TLR4/NF-kappa B signaling pathway Olfactory dysfunction |
| ISSN号 | 1567-5769 |
| DOI | 10.1016/j.intimp.2025.114189 |
| 通讯作者 | Chen, Yunhua(13701572197@163.com) ; Jiang, Yiming(jiangyiming@renji.com.com) ; Yu, Yafeng(wuyafeng@suda.edu.cn) |
| 英文摘要 | Objectives: Eosinophilic chronic rhinosinusitis (ECRS) is characterized by early, severe olfactory dysfunction and a high recurrence rate, with inadequate treatments. This article aims to elucidate the potential mechanisms by which luteolin may treat olfactory dysfunction in the context of ECRS. Methods: Thirty C57 BL/6 mice were randomly assigned to five groups: a control group and four experimental groups (ECRS, ECRS + Luteolin (Low, Medium, High Dose)). We conducted RNA sequencing, behavioral testing, ELISA, PCR, HE staining, Western blot analysis, and immunofluorescence staining. Additionally, human olfactory epithelial Cells (HOEPCs) were treated with Luteolin, TAK-242, QNZ, and Luteolin + LPS to investigate the underlying mechanisms. Results: Luteolin significantly reduced IL-4, IL-5, and IL-13 levels in the nasal lavage fluid (NLF) of ECRS mice, improving olfactory function and restoring OMP+ mature and Gap43(+) immature olfactory sensory neurons (OSNs). RNA sequencing revealed the involvement of the TLR4/NF-kappa B pathway in ECRS-related olfactory dysfunction and luteolin's therapeutic effects. Luteolin reduced TLR4+ cells, P65 nuclear translocation, and decreased protein levels of IL-1 beta, TLR4, MyD88, p-P65, P65, and p-P38. The treatment also lowered OSNs cell apoptosis by decreasing the levels of cleaved caspase-3 and caspase-9 levels, and increasing Bcl-2 protein. Antioxidant enzymes SOD, CAT, and GSH-Px were elevated, while MDA levels decreased. In ECRS HOEPCs, luteolin's anti-apoptotic effects on OSNs were reversed by LPS-induced TLR4/NF-kappa B activation. Conclusions: Luteolin ameliorates olfactory dysfunction associated with ECRS by modulating the TLR4/NF-kappa B signaling pathway. This modulation results in a reduction of TH2-type inflammation, oxidative stress, and apoptosis in OSNs. |
| WOS关键词 | ALLERGIC RHINITIS ; OXIDATIVE STRESS ; KAPPA-B |
| 资助项目 | Shanghai Municipal Health Com-mission[202040015] ; Science and Technology Bu-reau of Suzhou[SKYD2023106] ; Project of the First Affiliated Hospital of Soochow University[2100201] |
| WOS研究方向 | Immunology ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:001417933700001 |
| 出版者 | ELSEVIER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/316224]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Chen, Yunhua; Jiang, Yiming; Yu, Yafeng |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Safety Evaluat & Res, Shanghai, Peoples R China 2.Changshu Second Peoples Hosp, Dept Otorhinolaryngol, Suzhou, Jiangsu, Peoples R China 3.Shanghai Jiao Tong Univ, Ren Ji Hosp, Sch Med, Dept Otorhinolaryngol, Shanghai, Peoples R China 4.Soochow Univ, Affiliated Hosp 1, Dept Otorhinolaryngol, Suzhou, Jiangsu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Jiali,Dai, Li,Gu, Jun,et al. Luteolin alleviates olfactory dysfunction in eosinophilic chronic rhinosinusitis through modulation of the TLR4/NF-κB signaling pathway[J]. INTERNATIONAL IMMUNOPHARMACOLOGY,2025,148:15. |
| APA | Shi, Jiali.,Dai, Li.,Gu, Jun.,Liu, Lu.,Chen, Yunhua.,...&Yu, Yafeng.(2025).Luteolin alleviates olfactory dysfunction in eosinophilic chronic rhinosinusitis through modulation of the TLR4/NF-κB signaling pathway.INTERNATIONAL IMMUNOPHARMACOLOGY,148,15. |
| MLA | Shi, Jiali,et al."Luteolin alleviates olfactory dysfunction in eosinophilic chronic rhinosinusitis through modulation of the TLR4/NF-κB signaling pathway".INTERNATIONAL IMMUNOPHARMACOLOGY 148(2025):15. |
入库方式： OAI收割
来源：上海药物研究所
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