Nano-Polymers as Cas9 Inhibitors
文献类型：期刊论文
| 作者 | Chepurna, Oksana4; Chatterjee, Avradip3; Li, Yuanqing1,2; Ding, Hong2 ; Murali, Ramachandran3; Black, Keith L.4; Sun, Tao4
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| 刊名 | POLYMERS
 |
| 出版日期 | 2025-02-01 |
| 卷号 | 17期号:3页码:16 |
| 关键词 | CRISPR/Cas9 nano-polymer inhibitor polymalic acid polyglutamic acid polyaspartic acid trileucine |
| DOI | 10.3390/polym17030417 |
| 通讯作者 | Sun, Tao(tao.sun@cshs.org) |
| 英文摘要 | Despite wide applications of CRISPR/Cas9 technology, effective approaches for CRISPR delivery with functional control are limited. In an attempt to develop a nanoscale CRSIPR/Cas9 delivery platform, we discovered that several biocompatible polymers, including polymalic acid (PMLA), polyglutamic acid (PGA), and polyaspartic acid (PLD), when conjugated with a trileucine (LLL) moiety, can effectively inhibit Cas9 nuclease function. The Cas9 inhibition by those polymers is dose-dependent, with varying efficiency to achieve 100% inhibition. Further biophysical studies revealed that PMLA-LLL directly binds the Cas9 protein, resulting in a substantial decrease in Cas9/sgRNA binding affinity. Transmission electron microscopy and molecular docking were performed to provide a possible binding mechanism for PMLA-LLL to interact with Cas9. This work identified a new class of Cas9 inhibitor in nano-polymer form. These biodegradable polymers may serve as novel Cas9 delivery vehicles with a potential to enhance the precision of Cas9-mediated gene editing. |
| WOS关键词 | MECHANISMS ; CRISPR-CAS9 |
| 资助项目 | UCLA Clinical and Translational Science Institute (UCLA CTSI) Core Voucher[V182] ; UCLA Clinical and Translational Science Institute (UCLA CTSI) Core Voucher Award |
| WOS研究方向 | Polymer Science |
| 语种 | 英语 |
| WOS记录号 | WOS:001418383400001 |
| 出版者 | MDPI |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/316248]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Sun, Tao |
| 作者单位 | 1.Shenyang Pharmaceut Univ, Sch Tradit Chinese Mat Med, Shenyang 110016, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai 200231, Peoples R China 3.Cedars Sinai Med Ctr, Dept Biomed Sci, Res Div Immunol, Los Angeles, CA 90048 USA 4.Cedars Sinai Med Ctr, Dept Neurosurg, Los Angeles, CA 90048 USA |
| 推荐引用方式 GB/T 7714 | Chepurna, Oksana,Chatterjee, Avradip,Li, Yuanqing,et al. Nano-Polymers as Cas9 Inhibitors[J]. POLYMERS,2025,17(3):16. |
| APA | Chepurna, Oksana.,Chatterjee, Avradip.,Li, Yuanqing.,Ding, Hong.,Murali, Ramachandran.,...&Sun, Tao.(2025).Nano-Polymers as Cas9 Inhibitors.POLYMERS,17(3),16. |
| MLA | Chepurna, Oksana,et al."Nano-Polymers as Cas9 Inhibitors".POLYMERS 17.3(2025):16. |
入库方式： OAI收割
来源：上海药物研究所
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