Rubiadin Mediates the Upregulation of Hepatic Hepcidin and Alleviates Iron Overload via BMP6/SMAD1/5/9-Signaling Pathway
文献类型：期刊论文
| 作者 | Xie, Xueting4,5; Chang, Linyue2,3; Zhu, Xinyue4,5; Gong, Fengbei2,3; Che, Linlin4,5; Zhang, Rujun3; Wang, Lixin4,5; Gong, Chenyuan4,5; Fang, Cheng4,5; Yao, Chao4,5 |
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 出版日期 | 2025-02-01 |
| 卷号 | 26期号:3页码:14 |
| 关键词 | rubiadin hepcidin iron metabolism iron overload BMP6/SMAD1/5/9-signaling pathway |
| ISSN号 | 1661-6596 |
| DOI | 10.3390/ijms26031385 |
| 通讯作者 | Zhou, Yufu(zhouyufu@shutcm.edu.cn) ; Zhu, Shiguo(zhushiguo@shutcm.edu.cn) |
| 英文摘要 | Iron overload disease is characterized by the excessive accumulation of iron in the body. To better alleviate iron overload, there is an urgent need for safe and effective small molecule compounds. Rubiadin, the active ingredient derived from the Chinese herb Prismatomeris tetrandra, possesses notable anti-inflammatory and hepatoprotective properties. Nevertheless, its impact on iron metabolism remains largely unexplored. To determine the role of rubiadin on iron metabolism, Western blot analysis, real-time PCR analysis, and the measurement of serum iron were performed. Herein, we discovered that rubiadin significantly downregulated the expression of transferrin receptor 1, ferroportin 1, and ferritin light chain in ferric-ammonium-citrate-treated or -untreated HepG2 cells. Moreover, intraperitoneal administration of rubiadin remarkably decreased serum iron and duodenal iron content and upregulated expression of hepcidin mRNA in the livers of high-iron-fed mice. Mechanistically, bone morphogenetic protein 6 (BMP6) inhibitor LDN-193189 completely reversed the hepcidin upregulation and suppressor of mother against decapentaplegic 1/5/9 (SMAD1/5/9) phosphorylation induced by rubiadin. These results suggested that rubiadin increased hepcidin expression through the BMP6/SMAD1/5/9-signaling pathway. Collectively, our findings uncover a crucial mechanism through which rubiadin modulates iron metabolism and highlight it as a potential natural compound for alleviating iron-overload-related diseases. |
| WOS关键词 | FERROPORTIN ; EXPRESSION ; ANTHRAQUINONE ; MACROPHAGES ; BINDING |
| 资助项目 | Natural Science Foundation of Shanghai ; National Natural Science Foundation of China[82373908] ; National Natural Science Foundation of China[82404918] ; National Key R&D Program Strategic Scientific and Technological Innovation Cooperation Key Project[2022YFE0203600] ; Ministry of Science and Technology ; [22ZR1461600] ; [23ZR1460800] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:001418579200001 |
| 出版者 | MDPI |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/316251]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Zhou, Yufu; Zhu, Shiguo |
| 作者单位 | 1.Shanghai Univ Tradit Chinese Med, Sch Acupuncture Moxibust & Tuina, 1200 Cailun Rd, Shanghai 201203, Peoples R China 2.Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Nat Prod Res Ctr, Shanghai 201203, Peoples R China 4.Shanghai Univ Tradit Chinese Med, Ctr Tradit Chinese Med & Immunol Res, Sch Integrat Med, Shanghai 201203, Peoples R China 5.Shanghai Univ Tradit Chinese Med, Sch Integrat Med, Dept Immunol & Pathogen Biol, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xie, Xueting,Chang, Linyue,Zhu, Xinyue,et al. Rubiadin Mediates the Upregulation of Hepatic Hepcidin and Alleviates Iron Overload via BMP6/SMAD1/5/9-Signaling Pathway[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2025,26(3):14. |
| APA | Xie, Xueting.,Chang, Linyue.,Zhu, Xinyue.,Gong, Fengbei.,Che, Linlin.,...&Zhu, Shiguo.(2025).Rubiadin Mediates the Upregulation of Hepatic Hepcidin and Alleviates Iron Overload via BMP6/SMAD1/5/9-Signaling Pathway.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,26(3),14. |
| MLA | Xie, Xueting,et al."Rubiadin Mediates the Upregulation of Hepatic Hepcidin and Alleviates Iron Overload via BMP6/SMAD1/5/9-Signaling Pathway".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26.3(2025):14. |
入库方式： OAI收割
来源：上海药物研究所
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