A ready-to-use RNA delivery platform modulated by metal ions
文献类型：期刊论文
| 作者 | Qin, Zhaojian4; Jiang, Yongzhuo4; Tao, Peng4; Liu, Haifeng3; Zhong, Han4; Ding, Xiaoyi4; Zhao, Runze4; Lu, Yi3; Zhang, Lifeng4; Chen, Xiaomin4 |
| 刊名 | CHEMICAL ENGINEERING JOURNAL
 |
| 出版日期 | 2025-01-15 |
| 卷号 | 506页码:15 |
| 关键词 | RNA delivery Ready-to-use delivery Metallized-ionizable liposome |
| ISSN号 | 1385-8947 |
| DOI | 10.1016/j.cej.2025.160170 |
| 通讯作者 | Liu, Xiaolong(liux@sibcb.ac.cn) |
| 英文摘要 | RNA delivery platforms are important for both the experimental research and practical application of RNA therapeutics. Most existing delivery strategies depend on lipid-based nanoparticles (LNPs) produced by microfluidic devices. However, this encapsulation mode is not convenient for large-scale screening of RNAs and personalized treatment. Here, we present a ready-to-use mode for mRNA delivery based on empty liposomes stabilized by metal ions. The addition of metal ions, particularly Zn2+, can increase RNA loading (by similar to 1.5 to 2 times) and expression (by similar to 3 to 7 times). At the molecular scale, Zn2+ can reprogram the structure of the lipid membrane, ultimately forming a thicker and stiffer lipid membrane with a higher transition temperature of glass. Finally, metallized liposomes can remain stable at 4 degrees C for more than similar to 3 months or at room temperature for similar to 1 month. This ready-to-use platform will simplify experimental research and accelerate the development of RNA therapeutics. |
| WOS关键词 | MOLECULAR-DYNAMICS SIMULATIONS ; SYSTEM |
| 资助项目 | National Key R&D Program of China[2022YFA1103900] ; National Key R&D Program of China[2020YFA0509102] ; National Natural Science Foundation of China[32401275] ; National Natural Science Foundation of China[32470972] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB0980301] ; Zhejiang Provincial Natural Science Foundation of China[LHDMZ25H020001] ; Research Funds of Hangzhou Institute for Advanced Study, UCAS[2022ZZ01002] ; Research Funds of Hangzhou Institute for Advanced Study, UCAS[2023HIAS-V007] |
| WOS研究方向 | Engineering |
| 语种 | 英语 |
| WOS记录号 | WOS:001424148300001 |
| 出版者 | ELSEVIER SCIENCE SA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/316260]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Liu, Xiaolong |
| 作者单位 | 1.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Ctr Excellence Mol Cell Sci,Key Lab Multicell Syst, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Zhongshan Inst Drug Discovery, Shanghai Inst Mat Med, Zhongshan 528400, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Ctr Excellence Mol Cell Sci, Key Lab Multicell Syst, Shanghai 200031, Peoples R China 4.Univ Chinese Acad Sci, Key Lab Syst Hlth Sci Zhejiang Prov, Hangzhou Inst Adv Study, Sch Life Sci, Hangzhou 310024, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qin, Zhaojian,Jiang, Yongzhuo,Tao, Peng,et al. A ready-to-use RNA delivery platform modulated by metal ions[J]. CHEMICAL ENGINEERING JOURNAL,2025,506:15. |
| APA | Qin, Zhaojian.,Jiang, Yongzhuo.,Tao, Peng.,Liu, Haifeng.,Zhong, Han.,...&Liu, Xiaolong.(2025).A ready-to-use RNA delivery platform modulated by metal ions.CHEMICAL ENGINEERING JOURNAL,506,15. |
| MLA | Qin, Zhaojian,et al."A ready-to-use RNA delivery platform modulated by metal ions".CHEMICAL ENGINEERING JOURNAL 506(2025):15. |
入库方式： OAI收割
来源：上海药物研究所
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