A Novel Nitrite Reductase from Acinetobacter haemolyticus for Efficient Degradation of Nitrite
文献类型：期刊论文
| 作者 | Yin, Xiao-Yan2; Bonku, Emmanuel Mintah1; Yuan, Jian-Feng2; Yang, Zhong-Hua2 |
| 刊名 | BIOMOLECULES
 |
| 出版日期 | 2025 |
| 卷号 | 15期号:1页码:11 |
| 关键词 | nitrite reductase
|
| DOI | 10.3390/biom15010063 |
| 英文摘要 | Nitrite reductases play a crucial role in the nitrogen cycle, demonstrating significant potential for applications in the food industry and environmental remediation, particularly for nitrite degradation and detection. In this study, we identified a novel nitrite reductase (AhNiR) from a newly isolated denitrifying bacterium, Acinetobacter haemolyticus YD01. We constructed a heterologous expression system using E. coli BL21/pET28a-AhNir, which exhibited remarkable nitrite reductase enzyme activity of 29 U/mL in the culture broth, substantially higher than that reported for other strains. Structural analysis of AhNiR revealed the presence of [Fe-S] clusters, with molecular docking studies identifying Tyr-282 and Ala-289 as key catalytic sites. The enzymatic properties of AhNiR demonstrated an optimal pH of 7.5 and an optimal catalytic temperature of 30 degrees C. Its kinetic parameters, Km and vmax, were 1.53 mmol/L and 10.18 mmol/min, respectively, fitting with the Michaelis-Menten equation. This study represents the first report of a nitrite reductase from a denitrifying bacterium, providing a new enzyme source for nitrite degradation applications in the food industry and environmental remediation, as well as for biosensing technologies aimed at nitrite detection. |
| WOS关键词 | DENITRIFICATION ; NITRATE |
| 资助项目 | Scientific Research Foundation for the Returned Overseas Chinese Scholars (State Education Ministry) ; Key Project of Hubei Provincial Department of Science and Technology[2019AFB707] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001404626800001 |
| 出版者 | MDPI |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/315941]  |
| 专题 | 新药研究国家重点实验室 |
| 通讯作者 | Yang, Zhong-Hua |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 2.Zhejiang Normal Univ, Xingzhi Coll, Jinhua 321100, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yin, Xiao-Yan,Bonku, Emmanuel Mintah,Yuan, Jian-Feng,et al. A Novel Nitrite Reductase from Acinetobacter haemolyticus for Efficient Degradation of Nitrite[J]. BIOMOLECULES,2025,15(1):11. |
| APA | Yin, Xiao-Yan,Bonku, Emmanuel Mintah,Yuan, Jian-Feng,&Yang, Zhong-Hua.(2025).A Novel Nitrite Reductase from Acinetobacter haemolyticus for Efficient Degradation of Nitrite.BIOMOLECULES,15(1),11. |
| MLA | Yin, Xiao-Yan,et al."A Novel Nitrite Reductase from Acinetobacter haemolyticus for Efficient Degradation of Nitrite".BIOMOLECULES 15.1(2025):11. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。