中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 国家授时中心
  3. 中国科学院国家授时中心
  4. 量子频标研究室
Single Molecule Thermodynamic Penalties Applied to Enzymes by Whispering Gallery Mode Biosensors

文献类型：期刊论文

作者Houghton, Matthew C.3,4,5; Toropov, Nikita A.2,4,5; Yu, Deshui1; Bagby, Stefan3; Vollmer, Frank4,5
刊名ADVANCED SCIENCE
出版日期2024-07-12
页码9
关键词biosensors enzymes optical forces optoplasmonics peptides and proteins plasmonics
DOI10.1002/advs.202403195
英文摘要Optical microcavities, particularly whispering gallery mode (WGM) microcavities enhanced by plasmonic nanorods, are emerging as powerful platforms for single-molecule sensing. However, the impact of optical forces from the plasmonic near field on analyte molecules is inadequately understood. Using a standard optoplasmonic WGM single-molecule sensor to monitor two enzymes, both of which undergo an open-to-closed-to-open conformational transition, the work done on an enzyme by the WGM sensor as atoms of the enzyme move through the electric field gradient of the plasmonic hotspot during conformational change has been quantified. As the work done by the sensor on analyte enzymes can be modulated by varying WGM intensity, the WGM microcavity system can be used to apply free energy penalties to regulate enzyme activity at the single-molecule level. The findings advance the understanding of optical forces in WGM single-molecule sensing, potentially leading to the capability to precisely manipulate enzyme activity at the single-molecule level through tailored optical modulation. Turnover of enzymes bound to optoplasmonic sensors can be detected at the single-molecule level. By modulating the strength of the electric field generated by the plasmonic gold nanoparticle, optoplasmonic sensors may be capable of generating forces that oppose turnover and that could therefore be used to control enzymes at the single molecule level. Created with BioRender.com image
WOS关键词CONFORMATIONAL TRANSITIONS ; KINASE ; POLARIZABILITY ; SHIFT
资助项目UKRI BBSRC SWBio DTP[BB/T008741/1] ; UKRI BBSRC[BB/Y512977/1] ; UKRI EPSRC[EP/T002875/1]
WOS研究方向Chemistry ; Science & Technology - Other Topics ; Materials Science
语种英语
WOS记录号WOS:001266433300001
出版者WILEY
资助机构UKRI BBSRC SWBio DTP ; UKRI BBSRC SWBio DTP ; UKRI BBSRC ; UKRI BBSRC ; UKRI EPSRC ; UKRI EPSRC ; UKRI BBSRC SWBio DTP ; UKRI BBSRC SWBio DTP ; UKRI BBSRC ; UKRI BBSRC ; UKRI EPSRC ; UKRI EPSRC ; UKRI BBSRC SWBio DTP ; UKRI BBSRC SWBio DTP ; UKRI BBSRC ; UKRI BBSRC ; UKRI EPSRC ; UKRI EPSRC ; UKRI BBSRC SWBio DTP ; UKRI BBSRC SWBio DTP ; UKRI BBSRC ; UKRI BBSRC ; UKRI EPSRC ; UKRI EPSRC
源URL[http://210.72.145.45/handle/361003/14494]  
专题国家授时中心_量子频标研究室
通讯作者Houghton, Matthew C.; Vollmer, Frank
作者单位1.Chinese Acad Sci, Natl Time Serv Ctr, Xian 710600, Peoples R China
2.Univ Southampton, Optoelect Res Ctr, Southampton SO17 1BJ, England
3.Univ Bath, Dept Life Sci, Bath BA2 7AY, Somerset, England
4.Univ Exeter, Dept Phys & Astron, Exeter EX4 4QD, Devon, England
5.Univ Exeter, Living Syst Inst, Exeter EX4 4QD, Devon, England
推荐引用方式
GB/T 7714		 Houghton, Matthew C.,Toropov, Nikita A.,Yu, Deshui,et al. Single Molecule Thermodynamic Penalties Applied to Enzymes by Whispering Gallery Mode Biosensors[J]. ADVANCED SCIENCE,2024:9.
APA Houghton, Matthew C.,Toropov, Nikita A.,Yu, Deshui,Bagby, Stefan,&Vollmer, Frank.(2024).Single Molecule Thermodynamic Penalties Applied to Enzymes by Whispering Gallery Mode Biosensors.ADVANCED SCIENCE,9.
MLA Houghton, Matthew C.,et al."Single Molecule Thermodynamic Penalties Applied to Enzymes by Whispering Gallery Mode Biosensors".ADVANCED SCIENCE (2024):9.

入库方式： OAI收割

来源：国家授时中心

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。