High Efficiency Production of Functional Small Extracellular Vesicles through Cellular Self-Motivation
文献类型：期刊论文
| 作者 | Wang, Chen4; Zhao, Xinyu3; Yan, Tianhao2; Zhang, Shuxin4; Li, Xiaoru4; Chen, Ye4; Wu, Wenjie4; Qiao, Zihan4; Chen, Qiubo4; Wang, Xianwen5 |
| 刊名 | NANO LETTERS
 |
| 出版日期 | 2025-04-11 |
| 卷号 | 25期号:16页码:6471-6480 |
| 关键词 | Small extracellularvesicle Piezoionic hydrogel Traction force Cellular self-stimulation Stem cell therapy |
| ISSN号 | 1530-6984 |
| DOI | 10.1021/acs.nanolett.5c00094 |
| 通讯作者 | Chen, Xu-Lin(okcxl@126.com) ; Zhang, Qingchuan(zhangqc@ustc.edu.cn) ; Wu, Shangquan(wushq@ustc.edu.cn) |
| 英文摘要 | In stem cell therapies, small extracellular vesicles (sEVs) are extremely limited in application due to their limited production. Here, we propose a new concept of "cellular self-stimulation" and develop a cost-effective method for the preparation of sEVs, which enables the conversion of cellular traction to self-generated stimulation through piezoionic hydrogels and enhances the ability of cells to secrete sEVs by more than an order of magnitude. The traction of the adherent cells leads to deformation of the piezoionic substrate, which in turn translates into a millivolt-level electrical signal acting on the cell itself, stimulating the cell to produce more sEVs. These sEVs remain biologically intact and have shown excellent efficacy in in vitro and in vivo assays, confirming the superior therapeutic potential of high concentrations of sEVs. This provides a strong impetus for the development and dissemination of stem cell therapies. |
| 分类号 | 一类 |
| WOS关键词 | ELECTRICAL-STIMULATION ; SECRETION |
| 资助项目 | National Natural Science Foundation of China[12232017] ; National Natural Science Foundation of China[12222212] ; National Natural Science Foundation of China[12072339] ; National Natural Science Foundation of China[J2019-V-0006-0100] ; National Science and Technology Major Project |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:001465857900001 |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Science and Technology Major Project |
| 其他责任者 | Chen, Xu-Lin，Zhang, Qingchuan，Wu SQ |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/101034]  |
| 专题 | 力学研究所_流固耦合系统力学重点实验室(2012-) |
| 作者单位 | 1.Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech, Beijing 100190, Peoples R China 2.Jilin Univ, Coll Basic Med Sci, Dept Cell Biol & genet, Changchun 130021, Jilin, Peoples R China; 3.Anhui Med Univ, Affiliated Hosp 1, Dept Burns, Hefei 230022, Anhui, Peoples R China; 4.Univ Sci & Technol China, Dept Modern Mech, CAS Key Lab Mech Behav & Design Mat, Hefei 230027, Anhui, Peoples R China; 5.Anhui Med Univ, Anhui Prov Inst Translat Med, Res & Engn Ctr Biomed Mat, Sch Biomed Engn, Hefei 230032, Anhui, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Wang, Chen,Zhao, Xinyu,Yan, Tianhao,et al. High Efficiency Production of Functional Small Extracellular Vesicles through Cellular Self-Motivation[J]. NANO LETTERS,2025,25(16):6471-6480. |
| APA | Wang, Chen.,Zhao, Xinyu.,Yan, Tianhao.,Zhang, Shuxin.,Li, Xiaoru.,...&Wu SQ.(2025).High Efficiency Production of Functional Small Extracellular Vesicles through Cellular Self-Motivation.NANO LETTERS,25(16),6471-6480. |
| MLA | Wang, Chen,et al."High Efficiency Production of Functional Small Extracellular Vesicles through Cellular Self-Motivation".NANO LETTERS 25.16(2025):6471-6480. |
入库方式： OAI收割
来源：力学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。