Rapid and Sensitive Escherichia coli Detection: Integration of SERS and Acoustofluidics in a Lysis-Free Microfluidic Platform
文献类型：期刊论文
| 作者 | Park, Sohyun1; Kim, Kihyun1; Go, Anna2; Lee, Min-Ho2; Chen, Lingxin3; Choo, Jaebum1 |
| 刊名 | ACS SENSORS
 |
| 出版日期 | 2025-01-31 |
| 页码 | 11 |
| 关键词 | surface-enhanced Raman scattering acoustofluidics lysis-free bacteria on-chip detection |
| ISSN号 | 2379-3694 |
| DOI | 10.1021/acssensors.4c03118 |
| 通讯作者 | Chen, Lingxin(lxchen@yic.ac.cn) ; Choo, Jaebum(jbchoo@cau.ac.kr) |
| 英文摘要 | Bacterial infections, such as sepsis, require prompt and precise identification of the causative bacteria for appropriate antibiotics treatment. Traditional methods such as culturing take 2-5 days, while newer techniques such as reverse transcription-polymerase chain reaction and mass spectrometry are hindered by blood impurities. Consequently, this study developed a surface-enhanced Raman scattering (SERS)-based acoustofluidic technique for rapid bacterial detection without culturing or lysing. Target bacteria are first tagged with SERS nanotags in a microtube. The solution with tagged bacteria and unbound SERS nanotags is passed through a silicon microfluidic channel. A piezoelectric transducer generates acoustic waves within the channel, concentrating larger tagged bacteria in the center and pushing smaller unbound nanotags toward the channel walls. A laser beam is focused at the center of the channel, and the Raman signals of bacteria passing through the focal volume are measured for quantitative analysis. As a proof of concept, this study detected various concentrations of Escherichia coli at a limit of detection of 1.75 x 105 CFU/mL within 1 h. This method offers significant clinical potential, enabling rapid and accurate bacterial identification without genetic material extraction, cultivation, or lysis. |
| WOS关键词 | ENHANCED RAMAN-SCATTERING ; PATHOGENS ; SEPSIS |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:001411255100001 |
| 资助机构 | Ministry of Trade, Industry and Energy ; National Research Foundation of Korea ; Technology Innovation Program ; Ministry of Trade, Industry, and Energy (MOTIE, Korea) ; National Nature Science Foundation of China |
| 源URL | [http://ir.yic.ac.cn/handle/133337/40440]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 烟台海岸带研究所_山东省海岸带环境工程技术研究中心 |
| 通讯作者 | Chen, Lingxin; Choo, Jaebum |
| 作者单位 | 1.Chung Ang Univ, Dept Chem, Seoul 06974, South Korea 2.Chung Ang Univ, Sch Integrat Engn, Seoul 06974, South Korea 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Park, Sohyun,Kim, Kihyun,Go, Anna,et al. Rapid and Sensitive Escherichia coli Detection: Integration of SERS and Acoustofluidics in a Lysis-Free Microfluidic Platform[J]. ACS SENSORS,2025:11. |
| APA | Park, Sohyun,Kim, Kihyun,Go, Anna,Lee, Min-Ho,Chen, Lingxin,&Choo, Jaebum.(2025).Rapid and Sensitive Escherichia coli Detection: Integration of SERS and Acoustofluidics in a Lysis-Free Microfluidic Platform.ACS SENSORS,11. |
| MLA | Park, Sohyun,et al."Rapid and Sensitive Escherichia coli Detection: Integration of SERS and Acoustofluidics in a Lysis-Free Microfluidic Platform".ACS SENSORS (2025):11. |
入库方式： OAI收割
来源：烟台海岸带研究所
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