Assembly of allophycocyanin from individual subunits: involvement of apo-form in the formation of trimers
文献类型：期刊论文
| 作者 | Bodunova, Daria V.1; Gvozdev, Daniil A.1; Bukach, Olesya V.1; Sidorenko, Svetlana V.1; Perfilova, Kristina V.2; Yu, Li3; Qin, Song4; Sluchanko, Nikolai N.2; Ge, Baosheng3; Maksimov, Eugene G.1 |
| 刊名 | PHOTOSYNTHESIS RESEARCH
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| 出版日期 | 2025-04-01 |
| 卷号 | 163期号:2页码:14 |
| 关键词 | Allophycocyanin Trimer assembly Spectroscopy Protein biosynthesis Energy transfer |
| ISSN号 | 0166-8595 |
| DOI | 10.1007/s11120-025-01145-6 |
| 通讯作者 | Bodunova, Daria V.(darriabodunova@yandex.ru) |
| 英文摘要 | In cyanobacteria and red algae, allophycocyanin (APC), as well as other phycobiliproteins, is involved in the energy transfer of photosystems. Since APC is a potent fluorescent protein for imaging and biomedical applications, it is necessary to obtain purified protein in large quantities, which is currently possible by biosynthesis in bacterial systems. Here we emphasize the challenges of obtaining the trimeric form of the protein from alpha-APC and beta-APC subunits of allophycocyanin in vitro. This approach allowed us to study the individual subunits and to perform assembly of allophycocyanin trimers in vitro. Using different spectroscopic techniques, we detected the heterogeneity of the synthesized beta-APC and showed the possibility that not only holo-forms may be involved in trimer formation. Data allowed us to provide additional arguments in favor of excitonic coupling of chromophores in APC trimers. |
| WOS关键词 | ENERGY-TRANSFER ; BETA-PHYCOCYANIN ; C-PHYCOCYANIN ; CPCS-I ; PHYCOBILIPROTEINS ; LYASE ; PHYCOBILISOME ; PURIFICATION ; SPECTROSCOPY ; CHROMOPHORES |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:001446372300001 |
| 资助机构 | Russian Science Foundation ; International Partnership Program of Chinese Academy of Sciences ; Ministry of Science and Higher Education of the Russian Federation |
| 源URL | [http://ir.yic.ac.cn/handle/133337/40144]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 通讯作者 | Bodunova, Daria V. |
| 作者单位 | 1.Moscow State Univ, Fac Biol, Leninskye Gory 1,Build 12, Moscow 119234, Russia 2.Russian Acad Sci, AN Bach Inst Biochem, Fed Res Ctr Biotechnol, Leninsky Prospect 33-1, Moscow 119071, Russia 3.China Univ Petr Huadong, Coll Chem Engn, Qingdao 266580, Peoples R China 4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Bodunova, Daria V.,Gvozdev, Daniil A.,Bukach, Olesya V.,et al. Assembly of allophycocyanin from individual subunits: involvement of apo-form in the formation of trimers[J]. PHOTOSYNTHESIS RESEARCH,2025,163(2):14. |
| APA | Bodunova, Daria V..,Gvozdev, Daniil A..,Bukach, Olesya V..,Sidorenko, Svetlana V..,Perfilova, Kristina V..,...&Maksimov, Eugene G..(2025).Assembly of allophycocyanin from individual subunits: involvement of apo-form in the formation of trimers.PHOTOSYNTHESIS RESEARCH,163(2),14. |
| MLA | Bodunova, Daria V.,et al."Assembly of allophycocyanin from individual subunits: involvement of apo-form in the formation of trimers".PHOTOSYNTHESIS RESEARCH 163.2(2025):14. |
入库方式： OAI收割
来源：烟台海岸带研究所
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