Potential roles of LMNA in promoting longevity for larger Argopecten scallops
文献类型：期刊论文
| 作者 | Zhao, Yang1,3; Ning, Junhao1; Wang, Quanchao1; Liu, Guilong4; Xu, Xin4; Chen, Rongjie5; Wang, Chunde1; Lu, Xia2 |
| 刊名 | AQUACULTURE
 |
| 出版日期 | 2025-04-30 |
| 卷号 | 600页码:13 |
| 关键词 | Argopecten scallops LMNA-SIRT1 interaction RNA interference Longevity |
| ISSN号 | 0044-8486 |
| DOI | 10.1016/j.aquaculture.2025.742254 |
| 通讯作者 | Wang, Chunde(Chundewang2007@163.com) ; Lu, Xia(luxia_0202@163.com) |
| 英文摘要 | Bay scallops, Argopecten irradians, are a commercial bivalve species, but their small size, short longevity, and serious inbreeding depression make it difficult to further promote the farming of this species. Compared with bay scallops, some interspecific hybrids bay scallops and Peruvian scallops (A. purpuratus) have extraordinary growth advantages, such as longer longevity and greater sizes, which can be attributed to the longevity genes of Peruvian scallops (7-10 years). Lamin A/C (LMNA) is a nuclear scaffold protein that interacts with specific histone deacetylases (HDAC) to maintain their activity and regulate gene expression. LMNA mutations can lead to premature aging, and altered levels of LMNA are found in diverse diseases in most vertebrates; however, its roles in regulating senescence remains largely unknown in mollusk species. In this study, we cloned LMNA open reading frames (ORFs) from two closely correlated Argopecten scallops with different lifespans and explored their capacity to modulate longevity. One hundred and twenty-eight variations and one InDel of three nucleotides were discovered in ApLMNA and AiLMNA at the nucleotide sequence level, with 109 synonymous and 19 nonsynonymous SNPs, leading to 16 amino acid variations and the absence of S16 in AiLMNA. Tissue distribution analysis revealed that these two genes exhibited different trends during the development and aging of the two scallops. A yeast two-hybrid assays revealed a stronger interaction between LMNA and SIRT1 in A. purpuratus than in A. irradians. RNA interference (RNAi) of ApLMNA at 6 h significantly upregulated the expression of its downstream gene, SIRT1; however, SIRT1 was downregulated after RNAi of AiLMNA. The SIRT1-targeted genes FoxO, Mn-SOD, and CAT also showed different expression patterns. Transcriptome analysis revealed that the longevity pathways in Peruvian scallops were activated after RNAi. Overall, our study provides interesting avenues to understand the mechanism of longevity through LMNA genes and will help produce long-lived and large crossbred offsprings to maintain fisheries and aquaculture going. |
| WOS关键词 | LAMIN A/C ; GENE |
| WOS研究方向 | Fisheries ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:001422964100001 |
| 资助机构 | National Natural Science Foundation of China ; Earmarked Fund for Agriculture Seed Improvement Project of Shandong Province ; National Key Research and Development Program of China ; Scientific and Technological Project of Yantai, Shandong Province ; Earmarked Fund for Shandong Modern Agro-Industry Technology Research System |
| 源URL | [http://ir.yic.ac.cn/handle/133337/40367]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 海岸带生物资源高效利用研究与发展中心 |
| 通讯作者 | Wang, Chunde; Lu, Xia |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresour, Yantai 264003, Shandong, Peoples R China 2.Yantai Univ, Sch Ocean, Yantai 264005, Shandong, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Yantai Spring Sea AquaSeed Ltd, Yantai 264006, Shandong, Peoples R China 5.Laizhou Marine Dev & Fishery Serv Ctr, Laizhou 261400, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Yang,Ning, Junhao,Wang, Quanchao,et al. Potential roles of LMNA in promoting longevity for larger Argopecten scallops[J]. AQUACULTURE,2025,600:13. |
| APA | Zhao, Yang.,Ning, Junhao.,Wang, Quanchao.,Liu, Guilong.,Xu, Xin.,...&Lu, Xia.(2025).Potential roles of LMNA in promoting longevity for larger Argopecten scallops.AQUACULTURE,600,13. |
| MLA | Zhao, Yang,et al."Potential roles of LMNA in promoting longevity for larger Argopecten scallops".AQUACULTURE 600(2025):13. |
入库方式： OAI收割
来源：烟台海岸带研究所
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