The effect of glutathione metabolism on the release of Wound-Induced Spores from Pyropia yezoensis
文献类型：期刊论文
| 作者 | Chen, Haihong1; Shi, Ziyan1; Zhong, Zhihai2; Ji, Nanjing1; Zhou, Xiaoqian1; Dan, Zhijie1; Shen, Xin1 |
| 刊名 | ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
 |
| 出版日期 | 2025-03-01 |
| 卷号 | 86页码:12 |
| 关键词 | Glutathione metabolism Pyropia yezoensis Oxidative stress Wound-Induced Spores Asexual spore |
| ISSN号 | 2211-9264 |
| DOI | 10.1016/j.algal.2025.103921 |
| 通讯作者 | Chen, Haihong(chenhhhailey@163.com) ; Shen, Xin(shenthin@163.com) |
| 英文摘要 | Oxidative stress from mechanical injury can trigger the development of Wound-Induced Spores (WIS) in Pyropia yezoensis, yet the regulatory mechanisms underlying this process require further elucidation. In this study, comparative transcriptomic analysis between injured and uninjured P. yezoensis thalli revealed significant differential gene expression within the glutathione (GSH) metabolism pathway during WIS development. There was a down-regulation in the expression of genes encoding for gamma-glutamyl-cystein synthetase (GCL) and glutathione synthetase (GSS), which were pivotal for GSH biosynthesis, during the development of WIS. Moreover, a diminished expression level was also observed in genes associated with oxidative metabolism of GSH during this process, including those encoding glutathione S-transferase (GST), glutathione peroxidase (GPX), glutathione reductase (GR), ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR), and dihydroascorbate reductase (DHAR). However, this transcriptional regulation does not correspond to a significant decrease in intracellular GSH levels, which may be associated with a reduction in the oxidative metabolism of GSH, leading to decreased cellular utilization of GSH. Treatment injured P. yezoensis with Buthionine sulfoximine (BSO) promoted the release probability of WIS, the release efficiency of WIS and germination rate of asexual spores, while supplementation of GSH produced opposite results, suggesting that modulating GSH levels can regulate the release of WIS. Correlation analysis suggested that there was a significant positive correlation between the release of WIS and the germination rate of asexual spores, suggesting that promoting the release of WIS could lead to the acquisition of a greater number of asexual spore seedlings. Our findings underscore the key role of GSH metabolism in WIS regulation and provide a theoretical foundation for the subsequent development of strategies to obtain asexual spore seedlings. |
| WOS关键词 | REDOX HOMEOSTASIS ; OXIDATIVE STRESS ; BIOSYNTHESIS ; ASCORBATE ; PLANTS ; TOLERANCE ; GENES ; GSH ; ROS |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:001402704000001 |
| 资助机构 | Jiangsu Seed Industry Revitalization Project |
| 源URL | [http://ir.yic.ac.cn/handle/133337/40431]  |
| 专题 | 海岸带生物学与生物资源利用重点实验室 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 通讯作者 | Chen, Haihong; Shen, Xin |
| 作者单位 | 1.Jiangsu Ocean Univ, Jiangsu Key Lab Marine Bioresources & Environm, Jiangsu Key Lab Marine Biotechnol, Lianyungang 222005, Peoples R China 2.Chinese Acad Sci, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Haihong,Shi, Ziyan,Zhong, Zhihai,et al. The effect of glutathione metabolism on the release of Wound-Induced Spores from Pyropia yezoensis[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2025,86:12. |
| APA | Chen, Haihong.,Shi, Ziyan.,Zhong, Zhihai.,Ji, Nanjing.,Zhou, Xiaoqian.,...&Shen, Xin.(2025).The effect of glutathione metabolism on the release of Wound-Induced Spores from Pyropia yezoensis.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,86,12. |
| MLA | Chen, Haihong,et al."The effect of glutathione metabolism on the release of Wound-Induced Spores from Pyropia yezoensis".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 86(2025):12. |
入库方式： OAI收割
来源：烟台海岸带研究所
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