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Chemoenzymatic Synthesis of Highly O-Glycosylated MUC7 Glycopeptides for Probing Inhibitory Activity against Pseudomonas aeruginosa Biofilm Formation

文献类型：期刊论文

作者Ma, Wenjing2,3; Luo, Junyuan4,5; Liu, Huan1,2,3; Du, Qi6,7; Hao, Tianhui2,3; Jiang, Yinyu2; Huang, Zhengwei4; Lan, Lefu1,8; Li, Zhonghua6,7; Li, Tiehai2,3
刊名ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
出版日期2025-03-07
页码6
关键词Carbohydrates glycopeptide synthesis chemoenzymatic synthesis Pseudomonas aeruginosa biofilm inhibition
DOI10.1002/anie.202424312
通讯作者Lan, Lefu(llan@ucas.ac.cn) ; Li, Zhonghua(zhonghua_@fudan.edu.cn) ; Li, Tiehai(tiehaili@simm.ac.cn)
英文摘要MUC7, a highly glycosylated protein in saliva and respiratory tract, plays potential roles in facilitating bacterial clearance and preventing microbial invasion. The complexity of glycan structures and multiplicity of glycosylation sites of MUC7 make it very difficult to explore accurate biofunctions against pathogens. Here, we report an efficiently convergent chemoenzymatic approach to firstly synthesize highly O-glycosylated MUC7 glycopeptides with nine glycosylation sites bearing various glycoforms via the combined use of hydrophobic tag-assisted liquid-phase peptide synthesis and enzymatic-catalyzed glycan elongation. Biological evaluations reveal that different glycoforms of synthetic MUC7 glycopeptides mediate unique activities against biofilm formation of Pseudomonas aeruginosa, among which sialylated MUC7 glycopeptide exhibits better inhibitory activity and has the potential to develop antibacterial drugs.
WOS关键词SOLUTION-PHASE SYNTHESIS ; SOLID-PHASE ; LECTIN LECA ; RECEPTOR ; MUCINS ; DI
资助项目National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDB1060000] ; Shanghai Municipal Science and Technology Major Project, Shanghai Pujiang Program[22PJ1402000] ; National Key Research and Development Program of China[2023YFD1800100] ; Hangzhou Institute for Advanced Study, UCAS[2023HIAS-V006] ; [22377134] ; [22077130] ; [22307020]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:001439152200001
出版者WILEY-V C H VERLAG GMBH
源URL[http://119.78.100.183/handle/2S10ELR8/316526]  
专题中国科学院上海药物研究所
通讯作者Lan, Lefu; Li, Zhonghua; Li, Tiehai
作者单位1.Univ Chinese Acad Sci, Hangzhou Inst Adv Study, Sch Pharmaceut Sci & Technol, Hangzhou 310024, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Mat Med, Carbohydrate Based Drug Res Ctr, State Key Lab Chem Biol, Shanghai 201203, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Shanghai Jiao Tong Univ, Shanghai Peoples Hosp 9, Sch Med, Dept Endodont, Shanghai 200011, Peoples R China
5.Fudan Univ, Shanghai Stomatol Hosp, Shanghai 200001, Peoples R China
6.Fudan Univ, Zhongshan Hosp, Dept Biochem & Mol Biol, Sch Basic Med Sci,NHC Key Lab Glycoconjugates Res, Shanghai 200032, Peoples R China
7.Fudan Univ, Zhongshan Hosp, Liver Canc Inst, Shanghai 200032, Peoples R China
8.Anhui Med Univ, Affiliated Hosp 1, Anhui Prov Key Lab Infect Dis, Hefei 230022, Peoples R China
推荐引用方式
GB/T 7714		 Ma, Wenjing,Luo, Junyuan,Liu, Huan,et al. Chemoenzymatic Synthesis of Highly O-Glycosylated MUC7 Glycopeptides for Probing Inhibitory Activity against Pseudomonas aeruginosa Biofilm Formation[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,2025:6.
APA Ma, Wenjing.,Luo, Junyuan.,Liu, Huan.,Du, Qi.,Hao, Tianhui.,...&Li, Tiehai.(2025).Chemoenzymatic Synthesis of Highly O-Glycosylated MUC7 Glycopeptides for Probing Inhibitory Activity against Pseudomonas aeruginosa Biofilm Formation.ANGEWANDTE CHEMIE-INTERNATIONAL EDITION,6.
MLA Ma, Wenjing,et al."Chemoenzymatic Synthesis of Highly O-Glycosylated MUC7 Glycopeptides for Probing Inhibitory Activity against Pseudomonas aeruginosa Biofilm Formation".ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2025):6.

入库方式： OAI收割

来源：上海药物研究所

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