Multi-gene metabolic engineering of Pichia pastoris to synthesize ectoine
文献类型：期刊论文
| 作者 | Zhang, Shuai1,4,5; Cheng, Bingjie4,5; Liao, Qing4,5; Huang, Xuewu3; Mi, Mengjiao4; Huang, Ming4,5; Wu, Yue4,5; Wu, Shuyan2; Wang, Xiaoyuang5; Hu, Xiaoqing4,5 |
| 刊名 | JOURNAL OF BIOSCIENCE AND BIOENGINEERING
 |
| 出版日期 | 2025-05-01 |
| 卷号 | 139期号:5页码:347-353 |
| 关键词 | Pichia pastoris Ectoine ectABC Metabolic engineering L-Aspartate-beta-semialdehyde |
| ISSN号 | 1389-1723 |
| DOI | 10.1016/j.jbiosc.2025.02.006 |
| 通讯作者 | Hu, Xiaoqing(xiaoqinghu@jiangnan.edu) |
| 英文摘要 | As a promising osmolyte, ectoine has been widely applied in cosmetics, food, and pharmaceutical industries in recent years, therefore its biomanufacturer has attracted increasing interest. Ectoine-producing isolates were previously screened from halophilic microorganisms. After ectoine synthetase was identified, genetic engineering of Escherichia coli, Corynebacterium glutamicum, and Hansenula polymorpha were employed to produce ectoine. However, Pichia pastoris, another successful host capable of high-density cell culture, had not yet been exploited as an ectoinesynthesizing host. In this study, therefore, P. pastoris was employed for the first time to produce ectoine through multi-gene metabolic engineering. Firstly, Chromohalobacter salexigens HZS/E, a halophilic isolate producing 46.96 mg/ mL ectoine, was identified, while ectABC encoding ectoine synthetase was cloned. Later, ectABC was introduced into P. pastoris GS115 under the control of two different promoters. The results showed that PGAP-based HZS02 accumulated 8.03 g/L, 12.62 % higher than 7.13 g/L produced by PAOX-based HZS01. Finally, to enhance the supply of the precursor L-aspartate-b-semialdehyde, three genes (aspC, aK, and asD) were individually and collectively overexpressed. The highest ectoine yield was achieved at 10.88 g/L by GS115/pGAPZ A-ectABC-aspC-aK-asD. This study demonstrated that P. pastoris was a highly effective host for ectoine biosynthesis. (c) 2025, The Society for Biotechnology, Japan. All rights are reserved, including those for text and data mining, AI training, and similar technologies. |
| WOS关键词 | GENE-CLUSTER ; BIOSYNTHESIS ; CONSTRUCTION ; MILKING ; COLI |
| 资助项目 | National Key Research and Devel-opment Program of China[2021YFC210090 0] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:001461487200001 |
| 出版者 | SOC BIOSCIENCE BIOENGINEERING JAPAN |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/316959]  |
| 专题 | 中国科学院上海药物研究所 |
| 通讯作者 | Hu, Xiaoqing |
| 作者单位 | 1.Guangzhou Cn Ferment Biotechnol Co Ltd, Guangzhou 510550, Peoples R China 2.Massey Univ, Hopkirk Res Inst, AgResearch Ltd, Univ Ave & Lib Rd, Palmerston North 4442, New Zealand 3.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Safety Evaluat & Res, Shanghai 201203, Peoples R China 4.Jiangnan Univ, Sch Biotechnol, 1800 Lihu Ave, Wuxi 214122, Peoples R China 5.Jiangnan Univ, State Key Lab Food Sci & Resources, 1800 Lihu Ave, Wuxi 214122, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Shuai,Cheng, Bingjie,Liao, Qing,et al. Multi-gene metabolic engineering of Pichia pastoris to synthesize ectoine[J]. JOURNAL OF BIOSCIENCE AND BIOENGINEERING,2025,139(5):347-353. |
| APA | Zhang, Shuai.,Cheng, Bingjie.,Liao, Qing.,Huang, Xuewu.,Mi, Mengjiao.,...&Hu, Xiaoqing.(2025).Multi-gene metabolic engineering of Pichia pastoris to synthesize ectoine.JOURNAL OF BIOSCIENCE AND BIOENGINEERING,139(5),347-353. |
| MLA | Zhang, Shuai,et al."Multi-gene metabolic engineering of Pichia pastoris to synthesize ectoine".JOURNAL OF BIOSCIENCE AND BIOENGINEERING 139.5(2025):347-353. |
入库方式： OAI收割
来源：上海药物研究所
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